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tophat
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python and R
snpeff
python
tophat
r
RNAseq123
updated 2.7 years ago by
Kevin Blighe
★ 3.9k • written 2.7 years ago by
kusum
• 0
0
votes
1
reply
806
views
Is possible to use NOISeq with counts from RSubread?
noiseq
rsubread
tophat
bowtie
bowtie2
4.9 years ago
Marcelo Laia
▴ 450
0
votes
1
reply
883
views
Filtering Tophat/cufflinks data for GSEA with topGO
topGO
tophat
kolmogorov-smirnov test
updated 6.8 years ago by
James W. MacDonald
65k • written 6.8 years ago by
aaronrosenstein
• 0
2
votes
3
replies
2.1k
views
SystemPipeR: alignments with ambiguous pairing were dumped.
R
rnaseq
tophat
summarizeoverlaps
systempiper
updated 7.5 years ago by
Thomas Girke
★ 1.7k • written 7.5 years ago by
abass.conteh
• 0
2
votes
9
replies
7.5k
views
Multiple alignments (multi-mapped reads) and DESeq/edgeR pipeline
deseq
deseq2
edger
tophat
bowtie
8.9 years ago
ysdel
▴ 40
0
votes
3
replies
2.4k
views
Error when counting reads in genes with summarizeOverlaps (Genomic-Aligments package)
rnaseq
summarizeOverlaps
deseq2
tophat
genomicalignments
updated 9.4 years ago by
Dan Tenenbaum
★ 8.2k • written 9.4 years ago by
alejandro.colaneri
▴ 20
6 results • Page
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Answer: DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
by
Michael Love
41k
> I got many p values that had "1.000000e+00" and padj "1" and stat of "0" when I added the Log threshold of LFC >1 and LFC < -1...is this …
Comment: Trying to use enrichGO
by
fernanda.backsouza
▴ 10
With all my love, thank you Guido, GOxploreR beeing a big ally for me. I don't know how to be grateful right now.
Comment: DESeq2 output used for PCA plot on R studio
by
swbarnes2
★ 1.3k
You didn't generate it with an experiment, you made it up: Your PCA doesn't look like a good RNASeq experiment, because it's not.
Answer: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
swbarnes2
★ 1.3k
Your contrast is comparing LGR5 to Homeostasis. The bottom 2 are fine, it's the top one that is wrong. Are you sure this step isn't misla…
Comment: get BM error
by
James W. MacDonald
65k
I believe you need NCBI Gene IDs for KEGG, in which case you may need to map. The three genes you have shown here don't map, and of those t…
Votes
Answer: How to retrieve gene ontology GO class from gene list?
Answer: How to retrieve gene ontology GO class from gene list?
C: when to apply quantile normalization with voom in limma/voom framework with RNA-
A: How to know if I should use voomWithQualityWeights() or not?
A: Weird results (ribosomal proteins / Y-linked genes) in limma/voom
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