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Showing :
transcriptomics
•
reset
3
votes
2
replies
189
views
How to extract counts from a DESeq dataset ordered by a specific sample?
DESeq2
Transcriptomics
24 days ago
Anita
▴ 10
3
votes
5
replies
1.3k
views
DESeq2, time-course interpretation
Transcriptomics
TimeCourse
DESeq2
updated 9 months ago by
Michael Love
41k • written 9 months ago by
smac97
• 0
0
votes
4
replies
725
views
problem while retriving the genes by using p value and log2fc as conditions
Bioconductor
Transcriptomics
DifferentialExpression
DESeq2
11 months ago
sreeshmaraj72
• 0
0
votes
1
reply
337
views
Time change in expression vs time change in phenotype
Transcriptomics
ExpressionData
DESeq2
updated 12 months ago by
Michael Love
41k • written 12 months ago by
Ivan
• 0
4
votes
5
replies
1.6k
views
EnhancedVolcano - Unable to customize the length of Connectors to Gene Labels
Transcriptomics
EnhancedVolcano
updated 12 months ago by
Kevin Blighe
★ 3.9k • written 12 months ago by
Peter
▴ 20
4
votes
8
replies
1.3k
views
DESEQ2 - What is the recommended workflow in terms of pre-filtering and counts normalization?
DESeq2
Transcriptomics
updated 12 months ago by
Wolfgang Huber
★ 13k • written 12 months ago by
Peter
▴ 20
0
votes
3
replies
725
views
Understanding edgeR differential expression results
Transcriptomics
edgeR
DifferentialExpression
RNASeqData
RNASeq
updated 14 months ago by
Gordon Smyth
50k • written 14 months ago by
Teresa
• 0
0
votes
1
reply
701
views
Is it possible to convert transcript-level to gene-level dataset to use it in DESeq2 ?
DESeq2
Transcriptomics
tximport
deseq
txi
updated 15 months ago by
Dario Strbenac
★ 1.5k • written 15 months ago by
Hicham
▴ 10
0
votes
1
reply
1.2k
views
Variance stabilization transformation (VST), blind=TRUE
DESeq2
Transcriptomics
RNASeq
updated 15 months ago by
Michael Love
41k • written 15 months ago by
Jayesh Kumar
• 0
0
votes
0
replies
513
views
EdgeR without replication
Transcriptomics
16 months ago
kuttibiotech2009
• 0
0
votes
0
replies
537
views
Analysis of TIF-seq data to generate transcription start sites (TSS) and poly(A) site (PAS) clusters
Transcriptomics
SequencingData
17 months ago
Rom
• 0
0
votes
0
replies
583
views
Job:
EPA Postdoctoral Fellowship in Bioinformatics and Computational Toxicology
MethylSeq
Transcriptomics
Pathways
Epigenetics
RNASeq
17 months ago
whduke
• 0
0
votes
2
replies
679
views
Questions about `collapseReplicates` function in DESeq2
DESeq2
Transcriptomics
18 months ago
Yijing
• 0
0
votes
0
replies
509
views
WCGNA: Using Eigengenes when correlating modules to external features? What about PC2, PC3???
Network
Transcriptomics
MicrobiomeData
RNASEQ
WCGNA
19 months ago
Julieta
• 0
2
votes
1
reply
1.5k
views
Understanding tximport working with salmon outputs
Transcriptomics
tximport
updated 19 months ago by
ATpoint
★ 4.0k • written 19 months ago by
Florent
▴ 10
0
votes
3
replies
955
views
DESeq2 - transcript length correction necessary when quantifying reads from ends of transcripts?
Transcriptomics
DESeq2
21 months ago
marius.wenzel
• 0
4
votes
6
replies
1.4k
views
How to best visualize multi-level transcriptomics experiment data?
ggplot2
Transcriptomics
Visualization
R
limma
23 months ago
mohammedtoufiq91
▴ 10
1
vote
3
replies
1.0k
views
Identifying DEG between development stages
plant
Transcriptomics
DESeq2
r
updated 2.1 years ago by
Basti
▴ 770 • written 2.1 years ago by
cicely.day
• 0
2
votes
5
replies
1.4k
views
DESeq2 with baseline correction for gene expression differences
DESeq2
edgeR
Transcriptomics
updated 2.1 years ago by
Michael Love
41k • written 2.1 years ago by
R1337
• 0
2
votes
2
replies
803
views
Deseq2 Resul variation by LFC variation
rnaseqGene
DESeq2
Transcriptomics
2.5 years ago • updated 11 months ago
diangomaiga
• 0
1
vote
2
replies
1.3k
views
Not many DE genes using glmTreat
EdgeR
Bioinformatics
Transcriptomics
Quantseq
2.7 years ago
Barista
• 0
0
votes
0
replies
888
views
Job:
Senior Bioinformatician - University of Alabama at Birmingham
JobPosting
Transcriptomics
SingleCell
RNA-seq
2.7 years ago
lianov
• 0
0
votes
0
replies
807
views
Job:
[apply by 8/15] PhD scholarship on functional and evolutionary genomics
JobPosting
Transcriptomics
GenomicVariation
2.8 years ago
marie
• 0
0
votes
2
replies
937
views
Downloading org.Mm.eg.db after R reinstall
org.Mmu.eg.db
Transcriptomics
3.0 years ago
Kate
• 0
0
votes
0
replies
918
views
Consensus wgcna soft threshold for each data
Transcriptomics
WGCNA
wgcna
3.1 years ago
2323982403
▴ 60
0
votes
1
reply
749
views
Metatranscriptomics DESeq2 multifactorial sampling
Transcriptomics
DESeq2
updated 3.1 years ago by
Michael Love
41k • written 3.1 years ago by
seppe.demits
• 0
0
votes
0
replies
928
views
Job:
Bioinformatician at MiroBio, Oxford, UK
Transcriptomics
Bioconductor
Statistics
Job
Biocon
3.1 years ago
m.jackson
• 0
5
votes
5
replies
2.4k
views
analyzing RNA-Seq transcriptomic data (GEO accession: GSE96058)
GEO
RNASeqR
Transcriptomics
updated 3.2 years ago by
James W. MacDonald
65k • written 3.2 years ago by
H. Z. Amini
▴ 10
0
votes
0
replies
915
views
Job:
Computational Biologist at Denali Therapeutics
Transcriptomics
SingleCell
JobPosting
3.3 years ago
Steve Lianoglou
★ 13k
1
vote
1
reply
887
views
NanoStringDiff Not Recognized as Numeric
Bioconductor
Transcriptomics
NanoStringDiff
updated 3.5 years ago by
Laurent Gatto
1.6k • written 3.5 years ago by
Pedro
▴ 10
0
votes
0
replies
686
views
Problems with cummeRbund
plot
transcriptomics
differential expression
cummeRbund
4.1 years ago
raquelponti
• 0
1
vote
0
replies
945
views
News:
News: Bioinformatics Summer School - July 2020 - UCLouvain (Belgium)
rnaseq
course
proteomics
transcriptomics
scrnaseq
News
4.2 years ago
Laurent Gatto
1.6k
0
votes
4
replies
1.0k
views
GOexpress - Error when plotting expression data
RNA-seq
transcriptomics
gene ontology
gene expression
GOexpress
updated 4.2 years ago by
kevin.rue
▴ 350 • written 4.2 years ago by
atorson
• 0
0
votes
0
replies
852
views
Job:
Bioinformatics Scientist, CNS Transcriptomics at Alnylam Pharmaceuticals, Cambridge MA, USA
scRNA-seq
CNS
transcriptomics
RNAi
Job
4.3 years ago
Joseph Barry
▴ 10
0
votes
0
replies
756
views
Job:
Bionformatician in personalized oncotherapy (BEL)
job
bioinformatician
immunotherapy
genomics
transcriptomics
Job
4.4 years ago
cedric
• 0
1
vote
0
replies
850
views
Job:
Bioinformatics PhD: The circadian clock in the ageing plant, University of York, UK
transcriptomics
gene expression
time series
plant biology
circadian
Job
4.5 years ago
dezer
▴ 10
2
votes
2
replies
2.8k
views
How adjusted p-values are calculated in limma
limma
r
transcriptomics
FDR
statistics
updated 4.6 years ago by
Gordon Smyth
50k • written 4.6 years ago by
Wuschel
▴ 10
2
votes
0
replies
2.3k
views
Job:
Bioconductor infrastructure for the integration of genomics, transcriptomics and proteomics data
proteomics
genomics
transcriptomics
software
Job
4.6 years ago
Laurent Gatto
1.6k
1
vote
1
reply
908
views
cpm filtering parameter
cpm filtering
transcriptomics
rnaseq
edgeR
metatranscriptomics
updated 4.6 years ago by
Gordon Smyth
50k • written 4.6 years ago by
vm.higareda
▴ 10
0
votes
0
replies
916
views
Job:
Bionformatician Position / Heidelberg
neuroscience
data integration
transcriptomics
Job
5.1 years ago • updated 5.0 years ago
slezak
• 0
0
votes
0
replies
767
views
How to integrate and visualize RNASeq / Proteomics data for metabolomics pathways?
r
metabolomics
KEGG
Proteomics
Transcriptomics
5.1 years ago
Wuschel
▴ 10
1
vote
0
replies
1.6k
views
Job:
Full time and permanent research logistician in Bioinformatics
transcriptomics
proteomics
job
bioinformatician
Job
5.1 years ago
Laurent Gatto
1.6k
1
vote
1
reply
1.4k
views
News:
Bioinformatics Summer School - July 2019 - UCLouvain (Belgium)
RNAseq
deseq2
proteomics
transcriptomics
workshop
News
written 5.1 years ago by
Laurent Gatto
1.6k
3
votes
4
replies
1.3k
views
Writing a loop to clean up protein names
Proteomics
Transcriptomics
Loops
batch-processing
R
updated 5.5 years ago by
Sebastian Gibb
▴ 80 • written 5.5 years ago by
laural710
• 0
44 results • Page
1 of 1
Recent ...
Replies
Comment: Which input file is used for DGEList in EgdeR?
by
Michael Love
41k
"gene_counts_length_scaled.tsv file generated by the nf-core be exactly that?" No that is the counts scaled by the length. Counts + offset…
Comment: DESeq2 a lot of genes showing up as differentially expressed that only have 1 sa
by
Michael Love
41k
These genes are fine. They are just the ones where, by chance, the sample variance is below the sample mean. It happens. DESeq2 brings thes…
Comment: DEG with low counts
by
Michael Love
41k
These counts are too low, only a few samples above a count of 10. So for typical RNA-seq data that is underpowered to call differences. It…
Comment: DESeq filtering specific to contrasts
by
Michael Love
41k
If the counts are too low it in one group then you can't make the comparison either way (whether they are filtered out or just under-powere…
Comment: Estimating Cell Counts for EPIC V2 array data
by
normawiedman059
• 0
0 [103] spam_2.9-1 doParallel_1.0.17 iterators_1.0.14 [106] foreach_1.5.2 beeswarm_0.4.0 tgp_2.4-20 [109] beanplot_1.3.1 fpc_2.2-9 dbscan_1…
Votes
A: Docker container with bioconductor and devtools
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
Comment: DEXSeq DEXSeqDataSet count matrix duplication
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