Showing : voom • reset
0
votes
2
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162
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voom(calcNormFactors()) for normalization of RNAseq counts for WGCNA
Voom Limma WGCNA
16 days ago Gimena • 0
5
votes
2
replies
511
views
Filter genes by expression for voomLmFit
DEG edgeR limma voom filterByExpr
updated 4 months ago by 50k • written 4 months ago by • 0
7
votes
2
replies
735
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR limma voom RNASeq muscat
updated 4 months ago by 50k • written 4 months ago by • 0
3
votes
4
replies
513
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats r RNASeqData limma voom
4 months ago pl23 • 0
2
votes
4
replies
581
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom RNASeq limma
5 months ago Tyler Sagendorf ▴ 10
5
votes
3
replies
678
views
limma: More differentially expressed genes when factor included in model than when used as blocking factor
voom blocking limma
updated 6 months ago by 50k • written 6 months ago by ▴ 180
1
vote
1
reply
933
views
How to do GSEA over limma + voom DGE ?
GSEA voom limma
updated 9 months ago by 50k • written 9 months ago by • 0
1
vote
5
replies
1000
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI normalization limma voom Normalization
updated 9 months ago by 50k • written 9 months ago by • 0
0
votes
1
reply
442
views
How to do PFS after Voom + Limma DGE ?
RNASeqData limma voom PFS DGE
updated 9 months ago by 65k • written 9 months ago by • 0
0
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1
reply
584
views
Decidetest results summary different from results written as .csv file
edgeR voom decidetests DEG
updated 12 months ago by 50k • written 12 months ago by • 0
3
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4
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1.0k
views
Processing data with voom "expression values" and "weights"
limma voom
updated 15 months ago by 50k • written 15 months ago by • 0
1
vote
1
reply
554
views
Alternatives to Voom in experiments without replicate
limma edgeR Voom
updated 15 months ago by 50k • written 15 months ago by • 0
0
votes
3
replies
920
views
diagnosing shifted MD plots/biased DEGs despite filtering
edgeR voom normalization DifferentialExpression
updated 16 months ago by 50k • written 17 months ago by • 0
1
vote
1
reply
640
views
Voom variance values: Some minor confusion about which variance is fed to lowess when using a design matrix of covariates
voom
updated 17 months ago by 50k • written 17 months ago by • 0
1
vote
10
replies
2.0k
views
multiple voom runs with duplicateCorrelation and negative values in limma
limma voom
20 months ago • updated 19 months ago Hilary ▴ 30
2
votes
2
replies
1.2k
views
How to judge DEG analysis of these samples could be done by using limma::voom
limma Clustering voom RNA-Seq
21 months ago Yang Shi ▴ 10
2
votes
4
replies
1.3k
views
pre-ranked GSEA or not?
log2FC DESeq2 GSEA voom limma
updated 21 months ago by 50k • written 21 months ago by ▴ 10
5
votes
6
replies
1.7k
views
Removing the duplicated genes before limma::voom by using limma::avereps
RSEM avereps voom limma RNASeq
21 months ago Yang Shi ▴ 10
2
votes
4
replies
1.3k
views
Doing normalization when using voom for DEG analysis?
limma RSEM voom Normalization
21 months ago Yang Shi ▴ 10
2
votes
9
replies
2.2k
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA r RNASeqData voom limma
22 months ago Peipei • 0
2
votes
2
replies
1.4k
views
Handling NA values in limma's voom design matrix
limma voom design NAs
updated 23 months ago by 50k • written 23 months ago by ▴ 10
2
votes
4
replies
2.7k
views
Limma: How to deal with multiple batch effects and what is going on under the hood
limma voom
updated 24 months ago by 50k • written 24 months ago by ▴ 20
0
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3
replies
1.3k
views
limma voom contrasts
contrasts limma voom
updated 2.1 years ago by 50k • written 2.1 years ago by ▴ 90
0
votes
3
replies
981
views
Need help with a contrast design for limma with a missing group?
limma VOOM
updated 2.2 years ago by 50k • written 2.2 years ago by ▴ 50
3
votes
2
replies
1.4k
views
Multi-level design RRBS analysis with voomLmFit
edgeR voomLmFit limma duplicateCorrelation voom
2.4 years ago altintas.ali • 0
3
votes
5
replies
2.4k
views
limma-trend and voom results do not agree
limma limma-trend voom RNASeq
2.4 years ago Tyler Sagendorf ▴ 10
0
votes
2
replies
1.8k
views
Mean-variance trend and identifying significant DE with large amount of biological change in expression in time-series data
voom
2.4 years ago • updated 2.3 years ago hmviit • 0
0
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2
replies
1.3k
views
voom flips the sign relative to log(cpm)
limma limma-voom rnaseq voom
updated 2.6 years ago by 50k • written 2.6 years ago by • 0
0
votes
1
reply
1.8k
views
limma/voom for protein intensity data
limma proteomics voom
updated 2.6 years ago by ★ 4.0k • written 2.6 years ago by ▴ 70
0
votes
2
replies
953
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable voom contrasts
2.6 years ago James • 0
0
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2
replies
852
views
voom-limma workflow for RNAseq samples with very different numbers of counts
voom limma RNASeq
3.1 years ago annadv • 0
0
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2
replies
5.2k
views
TPM data in limma/voom
voom limma DifferentialExpression edgeR
3.2 years ago RJM • 0
1
vote
1
reply
1.3k
views
Limma-Voom - Time series experiment with one group (duplicateCorrelation() function)
voom duplicateCorrelation() timeseries limma
updated 3.3 years ago by 50k • written 3.3 years ago by ▴ 10
6
votes
13
replies
5.1k
views
How to use RSEM value to find differentially expressed genes
RSEM limma edgeR voom normalization
updated 21 months ago by 50k • written 3.7 years ago by ▴ 60
0
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5
replies
1.3k
views
isoform data, deep sequencing and voom
isoform deep sequencing voom
updated 3.7 years ago by 50k • written 3.7 years ago by ▴ 620
0
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4
replies
1.0k
views
mean-variance trend plots with isoform and gene-level data from tximport
tximport isoform voom
updated 3.8 years ago by 50k • written 3.8 years ago by ▴ 620
1
vote
4
replies
1.0k
views
How to compare a single RNA-Seq sample with microarray samples?
voom rna-seq microarray
updated 3.8 years ago by 65k • written 3.8 years ago by ▴ 10
0
votes
1
reply
787
views
Coefficients not estimable voom Lima with multiple numeric covariate
r limma design rnaseq voom
updated 3.8 years ago by 50k • written 3.8 years ago by • 0
4
votes
6
replies
2.6k
views
linear mixed models using edgeR+limma
linear mixed model edger limma voom RNA-seq
updated 3.9 years ago by 50k • written 3.9 years ago by ▴ 10
1
vote
5
replies
1.4k
views
Limma for proteomic data! Please Help!
limma voom lmfit ebayesfit
updated 4.0 years ago by 50k • written 4.0 years ago by • 0
0
votes
2
replies
648
views
estimating variability in absence of replicates for edgeR
edgeR RNAseq voom variability estimator
updated 4.1 years ago by 50k • written 4.1 years ago by • 0
3
votes
6
replies
1.7k
views
paired and unpaired test at the same time with voom limma
limma voom rna-seq paired test unpaired test
updated 4.1 years ago by 50k • written 4.1 years ago by • 0
7
votes
5
replies
2.4k
views
How to know if I should use voomWithQualityWeights() or not?
limma RNA-Seq voom voomWithQualityWeights edgeR
updated 4.1 years ago by 50k • written 4.1 years ago by ▴ 10
0
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0
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744
views
RNAseq/miRNAseq normalization for calculating gene correlations, survival analysis, and exploratory graphing
limma edger voom rnaseq mirnaseq
4.2 years ago atakanekiz ▴ 30
4
votes
7
replies
1.5k
views
Can I use (length bias normalization) from packages like EDAseq or cqn for input into VOOM?
limma voom edaseq edger rnaseq
updated 4.3 years ago by 50k • written 4.3 years ago by ▴ 50
3
votes
6
replies
3.1k
views
Understanding voom behavior when model matrix is used with/without intercept for a continuous variable
limma voom rnaseq model.matrix intercept
updated 4.4 years ago by 50k • written 4.4 years ago by ▴ 30
0
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0
replies
816
views
limma-voom group design and its effect on sva
limma voom group-design sva
4.4 years ago kentfung ▴ 20
2
votes
4
replies
1.2k
views
Limma gives big p-values for large LFC
limma voom
updated 4.5 years ago by 50k • written 4.5 years ago by • 0
4
votes
5
replies
943
views
Defining the design for a complicated multi-level experiment in Limma
limma RNASeq R voom
updated 4.5 years ago by 65k • written 4.5 years ago by ▴ 60
1
vote
1
reply
1.4k
views
Variance unstable after fitting to linear model with limma-voom pipeline
limma voom
4.5 years ago kentfung ▴ 20
50 results • Page 1 of 1
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Answer: Cannabis and KEGGgraph by deeenes ▴ 30
Hello, I'm a developer of the `OmnipathR` package, and I wrote [a function][1] to access certain KEGG data: library(OmnipathR) cs…
Answer: RNAseq:Technical replicates from different batches by Gordon Smyth 50k
I am not entirely clear what you mean by "technical" replicates in this case. Are they resequencing of the same RNA samples or are they fre…
Comment: DMR.Plot shows error "Error in simpleLoess(y, x, w, span, degree = degree, param by russ.bainer ▴ 10
Hi, I'm hitting this too. As far as I can tell my DataTrack is fine, but perhaps I am missing something. Notably the same code works on o…
Comment: The difference when using Limma compare between 3 conditions vs 2 conditions by Chris • 0
Hi Robert, If I don't want to use all pathway in c2 gene set but only a few pathways of interested, how can I do that? So we try to get a …
Comment: How to retrieve detailed regression results from deseq2 by Weiqian ▴ 10
Hi Michael, thanks for the answer! I'll have a look : )
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Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
A: Docker container with bioconductor and devtools
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
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