Showing : vst • reset
16
votes
12
replies
2.3k
views
VST transformation (DESeq2) for metabolomics data
vst Metabolomics DESeq2
2.0 years ago • updated 24 months ago Joan Miró ▴ 80
7
votes
11
replies
4.8k
views
Comparing means of VST tranformed RNA-Seq counts in different groups of samples with boxplots
VST deseq2 boxplot rnaseq
updated 5.9 years ago by 41k • written 5.9 years ago by ▴ 80
4
votes
9
replies
1.4k
views
Variance stabilising transformation to within given range
DESeq2 scaling vst
updated 3.2 years ago by ★ 13k • written 3.2 years ago by ▴ 40
4
votes
8
replies
22k
views
Variance stabilising transformation for machine learning
deseq2 vst rna-seq machine-learning
updated 4.7 years ago by 41k • written 4.7 years ago by ▴ 20
6
votes
6
replies
7.7k
views
DEseq2: Is vst/rlog transformation applied to raw counts or normalised counts?
deseq2 vst rlog transformation
updated 5.3 years ago by ★ 13k • written 5.3 years ago by ▴ 20
2
votes
6
replies
2.2k
views
Does LIMMA accept as an input "variance Stabilizing Transformation" generated by DSEQ
LIMMA VST
6.8 years ago • updated 6.5 years ago harelarik ▴ 60
4
votes
6
replies
3.5k
views
DESeq2 vst normalization: Do I have a heteroscedasticity problem and if so, what can I do about it?
deseq2 normalization rna-seq TCGA vst
updated 5.2 years ago by 41k • written 5.2 years ago by ▴ 70
6
votes
6
replies
2.3k
views
Transformation of raw RSEM gene expression estimates with variance stabilizing transformation and tximport R package
DESeq2 VST rsem tximport
3.4 years ago svlachavas ▴ 830
0
votes
6
replies
1.7k
views
Combine RNAseq datasets vst + Combat
combat vst deseq2
7.5 years ago User34591 • 0
4
votes
5
replies
1.1k
views
Difference in dimensionality reduction plots based on DESeq2 R package and core functions for RNA-Seq data
deseq2 vst pca cpm
4.2 years ago svlachavas ▴ 830
3
votes
5
replies
3.8k
views
Can I use VST on TPM data? (DESeq2)
DESeq2 VST
updated 2.4 years ago by 41k • written 2.4 years ago by ▴ 10
3
votes
5
replies
1.5k
views
VST transformation and covariates in the TCGA TARGET GTEx dataset
VST TCGATARGETGTEx DESeq2
updated 2.6 years ago by 41k • written 2.6 years ago by ▴ 10
1
vote
4
replies
1.7k
views
DESeq2 - VST data
DESeq2 VST
2.4 years ago AP • 0
2
votes
4
replies
2.5k
views
Is it valid to do gene comparisons on VST or rlog transformed data?
VST rlog
updated 2.3 years ago by • 0 • written 2.5 years ago by ▴ 10
0
votes
4
replies
2.8k
views
Normalization by variance stabilizing transformation VST
library composition vst DESeq2 Normalization
2.4 years ago kristoffersandas • 0
1
vote
4
replies
1.6k
views
plotCounts to visualize variations of a specific genes along conditions
DESeq2 plotCounts vst
updated 18 months ago by 41k • written 18 months ago by ▴ 10
0
votes
4
replies
1.3k
views
rlog fast=TRUE not correct?
deseq2 rlog transformation vst
7.9 years ago ldetorrente ▴ 10
4
votes
4
replies
2.0k
views
Unlog transformed data in DESeq2
RNASeq DESeq2 vst rlog Normalization
updated 4 months ago by 50k • written 2.4 years ago by ▴ 10
2
votes
4
replies
8.0k
views
Deconvolution with CIBERSORT: input data
normalization r vst cibersort
updated 3.7 years ago by ★ 3.9k • written 3.7 years ago by ▴ 60
6
votes
4
replies
3.7k
views
regularized log transformation- loss of zero values for sparsely expressed genes
deseq2 rlog transformation vst variancestabilizingtransformation rlog
updated 7.7 years ago by 41k • written 7.7 years ago by • 0
1
vote
3
replies
1.4k
views
Transformations of the variance in RNA-seq count data DESeq2
RNASeq vst R rlog DESeq2
updated 2.3 years ago by 41k • written 2.3 years ago by • 0
2
votes
3
replies
1.3k
views
Better to use rlog or vst transformation for data quality assessment of differental expression data based on size factors?
DESeq2 quality assesment vst rlog transformation
updated 5.9 years ago by 41k • written 5.9 years ago by ▴ 10
0
votes
3
replies
1.1k
views
Using transformed matrix in DESeq2
DESeq2 VST
updated 3.3 years ago by ★ 4.0k • written 3.3 years ago by • 0
0
votes
2
replies
733
views
Can VST-transformed community composition data be expressed as relative abundance?
vst relativeabundance Normalization DESeq2
10 months ago dwas • 0
5
votes
2
replies
1.4k
views
Is the VST implemented in DESeq useful on time-series cell differentiation datasets?
deseq deseq2 vst
updated 7.0 years ago by ★ 13k • written 7.0 years ago by • 0
2
votes
2
replies
830
views
Is the data after variance stabilising transformation (blind = FALSE) already corrected for covariates?
deseq2 vst covariates
updated 4.9 years ago by 41k • written 4.9 years ago by ▴ 20
3
votes
2
replies
1.1k
views
Variance stabilized values and batch correction
deseq2 vst
updated 3.7 years ago by 41k • written 3.7 years ago by ▴ 20
4
votes
2
replies
1.6k
views
VST Failing Error in estimateDispersionsFit(object, quiet = TRUE, fitType)
vst varianceStabilizingTransformation DESeq2
3.4 years ago Mia ▴ 10
0
votes
2
replies
1.4k
views
How to run vst transformation
RNASeqData vst
updated 23 months ago by 41k • written 23 months ago by • 0
0
votes
2
replies
793
views
Subsetting DESEQ2 VST Microbiome Data
DESeq2 16S VST
2.1 years ago vollkorrn • 0
1
vote
2
replies
1.0k
views
rlog vs vst for outlier identification
rlog DESeq2 vst outliers
2.3 years ago Bex • 0
2
votes
1
reply
1.0k
views
Converting vst-normalized gene expression counts back after correcting for tumor purity
Inverse-VST Tumor-Purity VST DESeq2 Normalization
updated 2.4 years ago by 41k • written 2.4 years ago by • 0
1
vote
1
reply
748
views
Unusal standard deviation after rlog transformation and the homoscedasticity of the data
rlog DESeq2 VST
updated 2.3 years ago by 41k • written 2.3 years ago by ▴ 10
0
votes
1
reply
591
views
GSA type of input data
GSEA GSA vst
updated 16 months ago by 41k • written 16 months ago by ▴ 30
0
votes
1
reply
2.7k
views
DESeq2: What is the unit of DESeq2 normalized read count (VST)? Is it tag per million?
deseq2 vst rlog
updated 4.5 years ago by 41k • written 4.5 years ago by • 0
0
votes
1
reply
877
views
Using VST normalized values for "external" single gene analysis
VST normalization deseq2
updated 4.7 years ago by 41k • written 4.7 years ago by • 0
0
votes
1
reply
829
views
Comparing transcriptome variation across groups after Variance stabilizing transformation using DESeq2
deseq2 vst variance
updated 4.9 years ago by 41k • written 4.9 years ago by • 0
0
votes
1
reply
743
views
Deseq2 Variance Stabalizing Transformation -- loss zeros to standard integer
deseq2 variancestabalizingtransformation vst
updated 5.1 years ago by 41k • written 5.1 years ago by • 0
1
vote
1
reply
996
views
Doubts about: Salmon -> Tximport -> Deseq2(vst) -> cox-regression
deseq2 vst cox-regression Salmon Tximport
updated 5.1 years ago by 41k • written 5.1 years ago by • 0
39 results • Page 1 of 1
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Comment: Opposite sign of LFC in count plots of DEGs (DESeq2) by winwater0928 • 0
Thank you for your comment! 1)"Your contrast is comparing LGR5 to Homeostasis." means (Lgr5)/(Homeostasis). Is it right? Then, I think if…
Answer: Reproducibility issue with scran together with lapply and BiocParallel by Aaron Lun ★ 28k
IIRC **BiocParallel** switches to a different RNG when inside its own functions like `bplapply` - specifically L'Ecuyer-CMRG, as opposed to…
Comment: DESeq2 output used for PCA plot on R studio by swbarnes2 ★ 1.3k
This looks terrible for RNASeq data, because it's not RNASeq data. It's a matrix of pretty random numbers, which you know because you poste…
Comment: DESeq2 output used for PCA plot on R studio by Aaliya • 0
I had taken this code from internet, but after reading about it and I did on my own, I would be making a post to ask for the suggestions if…
Comment: featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_ge by shuwangxinze1996 • 0
Hello, I used 48 cpus and 320G of memory to run this program. My bam files size are 7-9G, wonder how much CPU and memory is enough? Thank …
Votes
A: Are published RNA seq data analyses often wrong in calculating p-values and FDR?
A: How to explain how DESeq2 works to someone with zero bioinformatics background?
Answer: Interpret plot from DiffBind
Answer: How to retrieve gene ontology GO class from gene list?
Answer: How to retrieve gene ontology GO class from gene list?
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