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Comment: Transcript-level differential expression using DESeq2
by
Michael Love
36k
This is not an issue, see the vignette which talks about this phenomenon.
Answer: DESeq2, Calculate the size factor by using median-of-ratio
by
Michael Love
36k
I wouldn't recommend the original suggestion -- I'm not sure what exactly is the concern here, DESeq2 adapts to the higher dispersion in th…
Comment: EnrichGO not working with db created from annotationhub
by
Lucía
• 0
How can I find out the annotation file used by annotation hub? Like exactly what gtf/gff file and from where?
Comment: EnrichGO not working with db created from annotationhub
by
Lucía
• 0
Hi, thanks so much for your reply. This is the output of the commands you asked about: head( as.character(unique(annotated_significan…
Comment: Methods for removing unexpressed probes of Human Transcriptome Array HTA 2.0
by
Yang Shi
• 0
Perfect answer! This questions troubled me for a long time. Thanks again and for your quick and detailed reply.
Votes
Answer: Use MulticoreParam under BatchtoolsParam
Answer: Variance explained (coefficient of determination) in edgeR
HTSeq Counts no longer available
Comment: Problem converting transcript to genomic coordinates with Annotation Hub
Comment: Separate exogenous from endogenous transcripts using Salmon RNAseq DTU
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