User: Ben Mansfeld

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Posts by Ben Mansfeld

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Comment: C: GOSeq: analysis of unsupported genome after HTseq and DEseq2 - building gene len
... Thanks!  Any idea about the weird PWF plots? Why they are affected by filtering by log fold change? I read some other posts with similar issues but no conclusive response. -Ben ...
written 3.5 years ago by Ben Mansfeld0
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GOSeq: analysis of unsupported genome after HTseq and DEseq2 - building gene lengths, comparing contrasts and understanding results.
... Hello all, I am analyzing an RNAseq experiment in cucumber comparing two genotypes at two ages: Vlaspik (V) and Gy (G) at 8 and 16 days. I used HTseq-count for my read counts and subsequently performed DE analysis using DEseq2. Briefly: #Create DESeq data file from sample tabel, HTSeq data and m ...
rnaseq goseq deseq2 htseqcounts plant written 3.5 years ago by Ben Mansfeld0 • updated 3.5 years ago by Gordon Smyth39k

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