User: h.roest

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h.roest0
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Posts by h.roest

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Bugs in HTqPCR 1.30.0?
... Dear list, Tried to re-analyze an HTqPCR object from last year, but my limmaCtdata command in my R file failed where it worked OK last year. Running the R code that is coming with the package using R studio I noticed that certain modifications of the HTqPCR object "raw" results in the same probem w ...
htqpcr written 8 months ago by h.roest0
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Answer: A: No differential expression with limmaCtData possible
... Dear Axel, Thanks for your comment. Unfortunately, the "gnorm.raw.cat.Filt" object was created by filtering out the targets that were more then 4 out of 8 Undetermined. It looks that the problem actually starts here. limma of the unfiltered HTqPCR object can nicely pass the differential expression ...
written 8 months ago by h.roest0
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No differential expression with limmaCtData possible
... Dear All, Tried rerunning an R file that I made about a year ago to analyse TLDA array cards. However, when arriving at determining differentially expressed miRNAs I get an error where this returned a perfect set of differentially expressed miRNAs a year ago. sampleinfo<-read.delim("sampleinfo. ...
limma htqpcr written 9 months ago by h.roest0
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Answer: A: Possible bug in setCategory function from HTqPCR package
... Dear Matt, No answer, just the observation that the setCategory feature is not functioning properly. Tried to get in contact wit the package maintenance but no response yet. I (believe I) followed your R script to the letter which is supposed to be the solution to this problem. However, the Unrelia ...
written 22 months ago by h.roest0
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setCategory flag and flag.pout argument problems
... Hi all, I created an object of qPCRset class reading TLDA qPCR plates. I used three different characters to flag my Ct values; Passed, Flagged and Unreliable. All Undetermined and NA values are set to 40 in the qPCRset object. Using the setCategory in HTqPCR I tried to have those Ct values flagged ...
htqpcr written 22 months ago by h.roest0

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