User: Stefany Solano

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Posts by Stefany Solano

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Comment: C: Advice regarding RNAseq data with no replicates, best approach to accomplish fol
... Hi Aaron, I have another question. So regarding the model you suggested, thank you it does lower the BCV and the average log expression looks much much better. The thing is I'm a bit confused about how to write the contrast (I know you gave me the code but I definitely most be doing something wrong ...
written 9 weeks ago by Stefany Solano0
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Comment: C: Advice regarding RNAseq data with no replicates, best approach to accomplish fol
... Thank you very much Aaron, I'll try your suggestions now. The quality of the sequences are good and I've double check the pipeline I've used to mapped and to count the transcripts... I think the problem is the experimental design... anyway. Thank you very much! I've never asked anything before on th ...
written 9 weeks ago by Stefany Solano0
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Comment: C: Advice regarding RNAseq data with no replicates, best approach to accomplish fol
... Ok, just to be sure I'm on the same page as you, you mean to compare between time and condition isn't it? that's what you mean by gradients? to determine wether, let's say, at 24H there's a difference between fermenter Glu and fermenter Glu.FA. Is that what you mean? That' what I'm looking for as we ...
written 9 weeks ago by Stefany Solano0
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Answer: A: Advice regarding RNAseq data with no replicates, best approach to accomplish fol
... Hello Aaron, thank you very much!!! Ok following your nice explanation I'll proceed with the second strategy. Although I have some questions (I'm new to R and edgeR so I've learned basically using this package and looking up on internet how to do stuff, this said my understanding of how to code/inte ...
written 9 weeks ago by Stefany Solano0
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Advice regarding RNAseq data with no replicates, best approach to accomplish fold-change
...   Hello, I'm very sorry for posting again a question regarding RNA-seq with no replicates. I've read most of the posts and still don't know what's the best way to proceed. My experimental design was: two industrial fermenters, one as control, the other as the treatment and we took samples every 24 ...
rnaseq edger dge no replicates written 9 weeks ago by Stefany Solano0
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re-format data for HtqPCR
... Hi Hiedi, My name is Stefany I'm starting to learn R very recently so my expertise is limited. I've been reading your manual an some post from previous questions regarding how to re-format data to process with HTqPCR package. My data come from an Applied biosystems machine. I have to samples, two co ...
htqpcr reshape written 8 months ago by Stefany Solano0

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