User: linda.boshans

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Posts by linda.boshans

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Comment: C: Handling both technical and biological replicates in Ballgown
... Hi Jeff, Thank you for your response, but I don't quite understand. Could you please elaborate? How do I include the technical replicates as factors and how would that address the replicate issue? Do I assign the tech reps as a separate column in pData?  I have the following pData table:       id ...
written 7 months ago by linda.boshans0
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Handling both technical and biological replicates in Ballgown
... Hello, I am using the Hisat2 - Stringtie - Ballgown pipeline that was published in nature protocols in 2016.  When I upload my ballgown data in to r, I have 4 lane technical replicates per biological sample, in addition to 3 biological samples per condition (2 conditions total). This leads to 24 "s ...
biologicalreplicates technical replicates ballgown stringtie written 8 months ago by linda.boshans0 • updated 7 days ago by Alyssa Frazee110
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Comment: C: StringTie + Ballgown: handling biological replicates
... Hi Alyssa, I have a similar question to what was posted here, except I have 6 biological replicates (2 samples, 3 replicates each) and 4 technical replicates per biological replicates (for a total of 24). I have done as you stated for denoting the replicates in pData. How do I go about combining th ...
written 8 months ago by linda.boshans0
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Answer: A: StringTie + Ballgown: handling biological replicates
... Hi Alyssa, I have a similar question to what was posted here, except I have 6 biological replicates (2 samples, 3 replicates each) and 4 technical replicates per biological replicates (for a total of 24). I have done as you stated for denoting the replicates in pData. How do I go about combining th ...
written 8 months ago by linda.boshans0

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