User: taveluz

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taveluz0
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Posts by taveluz

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Comment: C: Two controls in RNAseq formula for DESeq2
... Hello Michael, Thank you very much for your response. I will look further advise on this as I agree this is not a trivial design. Bests, Tain. ...
written 4 days ago by taveluz0
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Comment: C: Two controls in RNAseq formula for DESeq2
... Yes, I noted that. I have no problem extracting the sex-specific differences using the `~group` design but instead trying to extract the general (i.e. no sex-specific) results for Drug vs DMEM. I think that what I am going to do is: 1. change my design to `~ sex + condition` set Vehiculo as contro ...
written 4 days ago by taveluz0
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Comment: C: Two controls in RNAseq formula for DESeq2
... Thank you for your suggestion. I have updated the question to state my problem more clearly. ...
written 7 days ago by taveluz0
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Comment: C: Two controls in RNAseq formula for DESeq2
... Hello @swbarnes2, I am already using the `results()` method to extract the contrast of interest, however, my concern is that I might be comparing, for instance, Drug vs DMEM using as control Vehicle (as this was the first level I specified in the formula) when in reality it must be Tibolone vs DMEM ...
written 7 days ago by taveluz0
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Two controls in RNAseq formula for DESeq2
... Hello, I have 5 treatments with 3 biological replicates and 2 technical replicates for each one. My design looks like: 1. DMEM (Basal condition) 2. Vehicle (Basal condition + vehicles used for treatments 3-5) 3. Drug 4. Insult 5. drug_insult After collapsing the technical reps my `sampleTab ...
deseq2 design formula controls written 7 days ago by taveluz0 • updated 6 days ago by Michael Love24k

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