User: abelsousa

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Posts by abelsousa

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gseaplot vertical line
... Hi, Does anyone know what the vertical line in the gseaplot from clusterProfiler represents? I saw in the help page that it indicates the maximum/minimal running enrichment score. But does anyone know more details about its calculation and meaning?   Cheers, Abel ...
clusterprofiler gsea written 7 months ago by abelsousa0 • updated 5 months ago by Bioconductor Community ♦♦ 0
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Comment: C: Removing batch effect from gene expression before Limma linear modelling
... Thank you Professor Gordon. In fact, I compared the results of one analysis where I included the batch factor in the linear model with other analysis where I removed the samples from project 2, and the genes that appear to be differentially expressed between tumour and normal are mostly the same. ...
written 12 months ago by abelsousa0
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Removing batch effect from gene expression before Limma linear modelling
... Hello,   I have a question about a differential expression analysis that I am doing using Limma on RNA-seq data. Basically, I want find differentially expressed genes between tumour and normal samples (in men and women separately), using as covariates ethnicity and age. To do so, I defined a mod ...
limma differential gene expression covariates batch effect correction written 12 months ago by abelsousa0 • updated 12 months ago by Gordon Smyth37k

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