User: damoros

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Posts by damoros

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Comment: C: Two-way anova 2x2 design with limma
... Thank you Aaron. I understand that the three t-statistic obtained with topTable in this case is equivalent to the three F statistics of aov function (and p-values). Two last questions: a)  In the case of a two-color analysis using the separate channels procedure, would this be the procedure to fol ...
written 3 days ago by damoros0
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Answer: A: Two-way anova 2x2 design with limma
... Hi. Thank you for your answers. I already have the latest version of user guide. I understand that the procedure in 9.5.2 is the recommended one (single factor), but I am required to perform two way anova. For example, if I extract nomalized intensities of one probe, I can perform a two way anova ...
written 4 days ago by damoros0
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Two-way anova 2x2 design with limma
... Hi, In the 8.7 section (Two-factor example) in limma User Guide, explains three procedures that give the same result. In the first procedure: > cont.matrix <- makeContrasts( + SvsUinWT=WT.S-WT.U, + SvsUinMu=Mu.S-Mu.U, + Diff=(Mu.S-Mu.U)-(WT.S-WT.U), + levels=design) > fit2 ...
microarray limma differential expression written 8 days ago by damoros0

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