User: dacaba.92

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dacaba.9210
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Posts by dacaba.92

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Comment: C: Limma PlotMDS: what values should be used?
... Many thanks Aaron, your answers are being so so useful for me. I have requested some information to the investigator in order to corroborate all my design. By the moment all it's clear to me,  unless the investigator says another different thing about the experiment design...Many thanks again Aaron. ...
written 23 months ago by dacaba.9210
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Comment: C: Limma PlotMDS: what values should be used?
... Sorry, I put you in context. Three cultures (A, B and C) were prepared from the same pure extract. Samples were collected from these cultures at different times, so for the first time we had samples 1A, 1B and 1C, for the second time 2A, 2B, 2C etc. All 36 samples were sequenced in the same run. Co ...
written 23 months ago by dacaba.9210
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Answer: A: Limma PlotMDS: what values should be used?
... Hi Aaron, many thanks for your quick response. I must apologize for one thing: MDS plots do actually vary due to the TMM normalization. The samples distribution is quite similar in both plots, and this is why I got mistaken, just didn't look enough to them.  Here both plots. First number means the ...
written 23 months ago by dacaba.9210
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Limma PlotMDS: what values should be used?
... Hi, I'm analyzing 36 RNAseq samples in order to obtain differentially expressed genes. The study design is a single time course with 12 timepoints ("t1" to "t12") and 3 replicates per timepoint (A, B and C) First of all I want to confirm the strategy I'm following it's the correct one. I'm using e ...
limma masigpro edger tmm plotmds written 23 months ago by dacaba.9210

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Popular Question 22 months ago, created a question with more than 1,000 views. For Limma PlotMDS: what values should be used?

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