User: cvu

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Comment: C: RNA-seq Batch Correction
... Thank you Aaron I am also inclined to believe that batch corrected data should not be used for differential expression analysis. Just had one counter-comment and an additional question If you could share your thoughts on these, that would be great. ================================================== ...
written 20 months ago by cvu0
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RNA-seq Batch Correction
... Hello All, I have an RNA-seq dataset and I've performed PCA analysis to check batch effect using package edgeR. and have used raw counts from cuffnorm output. here is my code     library(edgeR)     a1<-read.table("raw_counts.txt", sep="\t", header=TRUE)     dge1 <- DGEList(counts=a1[(-1)]) ...
limma edger rna-seq removebatcheffect() written 20 months ago by cvu0 • updated 20 months ago by Aaron Lun25k

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