User: martha.cooper

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Posts by martha.cooper

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Comment: C: Nanostring analysis with limma
... Thanks for your help James, and for replying so quickly! I am analysing a nanostring panel of 150 and as per your suggestion above, don't want to minimise biological differences by normalising to library size (or total count for nanostring). I thought that I could use limma-voom to calculate DEGs by ...
written 12 months ago by martha.cooper0
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Comment: C: Nanostring analysis with limma
... Hi James, I am currently trying to use voom() without normalising to library size, as you suggest in the above post.  As you mention above, I can specify a value for lib.size prior to running voom e.g. lib.size <- ... and then specific lib.size in the voom function e.g. voom(DGElist, design, ...
written 12 months ago by martha.cooper0

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