User: catpaw.tw

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catpaw.tw0
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Posts by catpaw.tw

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Comment: C: Comparison between limma and edgeR of reads ratio
... Hi Aaron,  Thanks so much for the suggestion, I will work on it. Just one more question, when you said "log-fold change", are you saying to use log-counts as input in edgeR? And, yes, you are correct, the total is the sum of ribosome bound plus the non-bound. The poly and total counts are correlate ...
written 4 weeks ago by catpaw.tw0
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Comment: C: Comparison between limma and edgeR of reads ratio
... Polysome profile is a technique used to study the mRNAs associated with ribosomes. The polysome fraction indicates the amount of mRNAs that is associated with ribosomes, and the total (whole polysome profiles) represents the total mRNAs amount (no matter they are free or engaged with ribosomes). It' ...
written 5 weeks ago by catpaw.tw0
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Comment: C: Comparison between limma and edgeR of reads ratio
... Hi Aaron,  Thanks for the comments, I have edited the original post. Let me know if anything is unclear. And yes, I understand that edgeR only takes raw counts, but under my case, I am worried that using raw counts will cause biases due to tech variations among fractions reads. For example, one fra ...
written 5 weeks ago by catpaw.tw0
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Comparison between limma and edgeR of reads ratio
... Hi All,  Thanks in advance for the help! I am still new to edgeR and welcome for any suggestions! I am trying to figure out which method is better off to analyze my data. I am interesting in translational change before vs. after stress in yeast(s. cerevisiae). I carried out polysome profiles befor ...
limma edger offset written 5 weeks ago by catpaw.tw0 • updated 4 weeks ago by Gordon Smyth33k

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