User: kjkjindal

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kjkjindal0
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Posts by kjkjindal

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Comment: C: How to simulate time course RNA seq data using DESeq?
... Hi Micheal, thanks for pointing me towards the relevant link(s). I had a follow up question about this and felt it would be more appropriate to ask it here rather than as a separate question. I checked the source code of the makeExampleDESeqDataSet function. I see that you pass mu=mu and size = 1/d ...
written 11 weeks ago by kjkjindal0
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Comment: C: How to simulate time course RNA seq data using DESeq?
... Indeed, what I am trying to do is simulate multiple time courses of count data for a gene, all originating from the same ground truth (true transcript level time course for the gene). Therefore, I thought it would be a good idea to sample from the NB distribution NB(mu_ij, alpha_i) for the i th gene ...
written 3 months ago by kjkjindal0
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How to simulate time course RNA seq data using DESeq?
... Hi all, I am trying to use DESeq2 to simulate time course RNA seq dataset. Setup details: Let's assume we have a time course dataset with 1000 genes, 8 time points and 3 replicates per time point. Here each time point is a 'condition' amounting to a total of 8 conditions. The total number of samp ...
deseq deseq2 R makeexampledeseqdataset written 3 months ago by kjkjindal0 • updated 3 months ago by Michael Love19k

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