User: Biomed

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Biomed0
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Posts by Biomed

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Comment: C: Help for Affymetrix HTA 2.0 [transcript (gene) version] annotation
... I'm so sorry for my careless! It's weird that I really add the reply in English! Apologize again! Here is my formal answer: Thank you! Svlachavas. Obviously, you are experienced in HTA 2.0. I have some other questions. Can you tell me the correct answer? Q1, because the oligo can only support th ...
written 15 months ago by Biomed0
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Comment: C: Help for Affymetrix HTA 2.0 [transcript (gene) version] annotation
... eSet=read.delim("GSE73219_series_matrix.txt") 昏暗(eSet)#[1] 70523 13 rownames(eSet)=eSet$ID_REF eSet=eSet[,-1] ESET=new("ExpressionSet", exprs=as.matrix(eSet)) ##limma DEGs filtering cell_line=factor(rep(c("Hep3B","Huh1"),each=6)) treat=factor(rep(c("scr","RDBP"),each=3,times=2)) combine=paste(cel ...
written 15 months ago by Biomed0
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Comment: C: Help for Affymetrix HTA 2.0 [transcript (gene) version] annotation
... Thank you! Svlachavas. Obviously, you are experienced in HTA 2.0. I have some other questions. Can you tell me the correct answer? Q1, because the oligo can only support the pretreatment process of the oligonucleotide microarray, can I start the analysis directly with the GEO series Metrix files? ...
written 15 months ago by Biomed0
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Help for Affymetrix HTA 2.0 [transcript (gene) version] annotation
... Hi all, It's my first-time to analysis Affymetrix HTA 2.0 arrays [transcript (gene) version], data source https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi. As a rookie, I have been bothered by its annotation for a long time, really hoping there would be someone teach me something. Thank you in advanc ...
annotation transcripts hta2.0 written 15 months ago by Biomed0 • updated 15 months ago by svlachavas740
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Comment: C: Why my limma DEG result in top Table all without significant meaning,showing log
... Thanks, Gordon, and very sorry for my rude action. It's true that I didn't get any result. Maybe there are some problems with my data before limma. I will check it first. Thanks again!   ...
written 15 months ago by Biomed0
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Why my limma DEG result in top Table all without significant meaning,showing logFC <1.5 or logFC>-1.5 ?
... Hi, all I'm dealing with Agilent single channel microarray when I filtering DEGs using limma, I find all my results showing logFC>-1.5 or logFC<1.5, without significant expression. I have been searching reason for a long time, but I still don't know what my faults are, I really need some help ...
limma toptable logfc deg written 15 months ago by Biomed0 • updated 15 months ago by Gordon Smyth39k
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Comment: C: Analysing Agilent single color array
... Hi,Li, I'm trying to analysis Agilent single channel microarray with Agi4x44PreProcess, but it always informs me that it can only work for RGList, not EList. Anything I missed? Look forward to your reply. Thanks! ...
written 15 months ago by Biomed0

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