User: hamza_karakurt

Reputation:
30
Status:
New User
Location:
Last seen:
5 days, 16 hours ago
Joined:
1 year ago
Email:
h*************@windowslive.com

Posts by hamza_karakurt

<prev • 24 results • page 1 of 3 • next >
2
votes
1
answer
67
views
1
answer
Batch Corrections (MNN) with TPM values
... Hello, I have a simple question. I have 2 public data sets and I want to use both of them with MNN correction for certain analyses. One of the data sets has raw counts, which is suitable to use in Scater/Scran but the other one only has TPM values in the supplementary. To use MNN we need normalized ...
rna-seq scran scater scrna-seq mnn written 6 days ago by hamza_karakurt30 • updated 6 days ago by Aaron Lun25k
0
votes
0
answers
99
views
0
answers
emptyDrops result is full NAs
... Hello, I am trying to use emptyDrops function of dropletUtils package. I used it on my raw counts data but the results is full of NAs. How can I solve this problem or what can be wrong about my data? Thank you in advance. My sessionInfo: R version 3.5.2 (2018-12-20) Platform: x86_64-apple-darwin ...
scran scater dropletutils scrna-seq written 4 months ago by hamza_karakurt30
0
votes
1
answer
335
views
1
answers
Comment: C: Differential Expression Analysis between conditions of single cell RNA-Seq
... Hello again Aaron, Micheal and thank you for answers. Counting counts and create a pseudo-bulk RNA-Seq is an effective method I see and it is suitable to use on specific cell types but I face another question mark. What if we don't have replicates which mean only 1 data for healthy and 1 data for di ...
written 6 months ago by hamza_karakurt30
0
votes
1
answer
195
views
1
answers
Comment: C: Usage of doubletCells() function before or after the batch correction?
... Thank you Aaron, You approach looks really useful and makes sense. I was thinking the same but just wanted to be sure. After computing doublet scores for each data, I will merge the scores to create a vector (same length as cell number) and assign them into the corrected SingleCellExperiment object ...
written 6 months ago by hamza_karakurt30
4
votes
1
answer
195
views
1
answer
Usage of doubletCells() function before or after the batch correction?
... Hello, I am doing a scRNA-Seq analysis and I want to use doubletCells() function to identify possible doublets. My data comes from 4 different batches and I use fastMNN for batch correction. Which way would be better in this situation? Using doubletCells() for each data before batch correction and ...
scran scater scrna-seq doubletcells written 6 months ago by hamza_karakurt30 • updated 6 months ago by Aaron Lun25k
0
votes
1
answer
243
views
1
answers
Comment: C: Using SC3 with batch corrected MNN values
... Thank you for answer. Actually I am planning to use MNN correction. It is more suitable in my situation and further analyses I am planning. MNN can create a corrected expression matrix but it also have negative values (due to cosine normalization I believe). I took the risk and used SC3 on this corr ...
written 6 months ago by hamza_karakurt30
0
votes
1
answer
243
views
1
answers
Comment: C: Using SC3 with batch corrected MNN values
... Thank you for answer. As you said, negative values effects results as expected. To try it, I used sc3_estimate_k function on both data set itself and reduced dimension (PCA with first 50 PCs in that case), and estimated k was 27 for all data and 5 for reduced dimension. Probably it is not a good way ...
written 6 months ago by hamza_karakurt30
4
votes
1
answer
243
views
1
answer
Using SC3 with batch corrected MNN values
... Hello, I want to use SC3 for data sets from multiple batches. I use fastMNN() function of Scran/Scater package for batch normalization but it does not effect logcounts, it creates a reduced dimension "MNN" that shows the corrected data which also used in clustering step. How can I use SC3 with these ...
scran scater sc3 scrna-seq batch correction written 6 months ago by hamza_karakurt30 • updated 6 months ago by Vladimir Kiselev150
1
vote
1
answer
233
views
1
answer
Dunn Index for Clusters from scRNA-Seq
... Hello everyone, I want to try Dunn Index to validate my clustering results from scRNA-Seq data. I know the Dunn index starts from 0 and goes to infinity and higher results mean better clustering. I tried this method from scRNA-Seq which clustered with buildSNNGraph() function of Scater/Scran package ...
clustering scran scater scrna-seq dunn index written 7 months ago by hamza_karakurt30 • updated 7 months ago by Aaron Lun25k
5
votes
1
answer
335
views
5 follow
1
answer
Differential Expression Analysis between conditions of single cell RNA-Seq
... Hello everyone, I am working on scRNA-Seq data analysis and I have a technical question. We can combine different scRNA-Seq experiments with batch correction methods such as MNN or CCA. As I know, while doing differential expression analysis we should consider batch effect like Scater/Scran package ...
deseq2 rna-seq scrna-seq differential expression analysis written 7 months ago by hamza_karakurt30 • updated 7 months ago by Michael Love25k

Latest awards to hamza_karakurt

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 206 users visited in the last hour