User: newbio17

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newbio170
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Posts by newbio17

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Comment: C: How to set up appropriate analysis using sample type and condition for DESeq2
... Hi Michael, For comparisons within each time point, could I employ GFOLD which doesn't require replicates to perform DEG? Would it be not good practice to report findings from two different tools? ...
written 11 months ago by newbio170
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Comment: C: How to set up appropriate analysis using sample type and condition for DESeq2
... I see. It was my understanding that I would need more than one sample belonging to each group (in this example, 3 cell-AC, 3 cell-AT, etc... since 3 is what I believe is the minimum for cell lines). ...
written 12 months ago by newbio170
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Comment: C: How to set up appropriate analysis using sample type and condition for DESeq2
... Thank you, Michael! Is it true that the newer releases for DESeq2 will now throw an error when you try to run DESeq without replicates? ...
written 12 months ago by newbio170
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How to set up appropriate analysis using sample type and condition for DESeq2
... Note: I do not have biological replicates. I have read the warning messages and am aware that the analysis without replicates probably will not yield any meaning results. I want to make sure that I have the concept down for future reference. What is the appropriate way of performing differential ex ...
deseq2 design differential gene expression written 12 months ago by newbio170 • updated 12 months ago by Michael Love25k

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