User: chiara.facciotto

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Answer: A: Data normalization and transformation using DESeq2
... Thanks! And is it ok to run estimateSizeFactors(dss) before running rlog(dds, blind=FALSE)? ...
written 8 days ago by chiara.facciotto0
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Data normalization and transformation using DESeq2
... Hi, I am trying to use DESeq2 to perform normalization of my RNA-Seq data. I am wondering if, after estimating the size factors, I can directly run the rlog or vst transformation, or if I also need to use the count function in between. Basically I wonder which is the correct one between option 1 or ...

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