User: lornaas

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lornaas0
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Posts by lornaas

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Order of design matrix for edgeR analysis
... I'm running an edgeR analysis on RNAseq data that was run in two seperate batches which I want to control for. Should the variable I'm interested in be first or last in the equation? ie "design <- model.matrix(~group + batch)" or "design <- model.matrix(~batch + group)"? The edgeR manual ...
edger R written 7 weeks ago by lornaas0 • updated 7 weeks ago by James W. MacDonald51k
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How important is lfcSE?
... I have carried out a DESeq2 analysis on a relatively small data set (8v8) and have been going through the results. A large number of genes have come out as statistically different, however a very small number of these genes seem to have a very large lfcSE (between 0.6 and 1.5). Even though they are ...
deseq2 written 9 months ago by lornaas0 • updated 9 months ago by Michael Love26k

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