User: marie
marie • 0
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Posts by marie
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... I see. Thank you very much for your response. Yes, it could be possible; there can be a huge difference between the condition. I will discuss with my collaborators and will also search for studies with a similar setting.
Actually, this is my first experience observing such an extreme diversity betwe ...
written 3 months ago by
marie • 0
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... The actual data is like this:
```sample stage1_treatment1 stage1_treatment2 stage1_treatment2 stage1_treatment3 stage1_treatment2 stage1_treatment3 stage1_treatment2 stage1_treatment1 stage1_treatment3 stage1_treatment3 stage1_treatment3 stage1_treatment3 stage1_treatment1 stage2_treatment1 stage2_ ...
written 3 months ago by
marie • 0
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... Thank you for the prompt response.
I tried `DESeqDataSetFromTximport` this time, but the last problem remains.
**What I was trying to ask is:**
I expected the sum of all gene expression in the tximport output is same across the samples, like some other normalization methods, e.g., "all TPMs (trans ...
written 3 months ago by
marie • 0
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... Hi, I am trying to do comparative RNA-seq analysis with DESeq2.
My purposes are:
**1. combine transcripts into genes
2. detect gene expression difference under different conditions
3. obtain a summarized gene expression table**
1. combine transcripts into genes
I basically followed these commands ...
written 3 months ago by
marie • 0
• updated
3 months ago by
Michael Love ♦ 24k
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