User: domenicoalessandrosilvestris

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Posts by domenicoalessandrosilvestris

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Comment: C: DESeQ2 design multiple conditions
... From what I understand "contrast" extracts only the results related to the comparison you are requesting, but the actual statistical test is performed by the "DESeq" function in fact when I go to open the dds it tells me "assays (6)". Actually the problems are two: to avoid making thousands of usele ...
written 5 months ago by domenicoalessandrosilvestris0
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Comment: C: DESeQ2 design multiple conditions
... Thanks for the reply! I know that your solution is obviously the simplest but in that way when you extract the normalized counts you get slightly different values ​​for the same gene in the same sample depending on the comparison you are considering and if you have to use these values ​​for example ...
written 5 months ago by domenicoalessandrosilvestris0
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DESeQ2 design multiple conditions
... Hi, I'm using DESeq2 with 4 multiple conditions ("A", "B", "C" and "D"), but I'm **only** interested in the comparisons A vs C; A vs D; B vs C and B vs D. Since the higher the number of tests performed and the lower the significant results (and the "contrast" argument of the result function perform ...
deseq2 written 5 months ago by domenicoalessandrosilvestris0 • updated 5 months ago by swbarnes2330

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