User: gene_bioconductor

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Posts by gene_bioconductor

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Reproducing FlowJo Biexponential Transform in flowCore
... I'm trying to reproduce a FlowJo workflow in flowCore. I received a description of the FlowJo biexponential transform settings from my colleague, which are: # Minimum is -1622.427; -10^3, no extra negatives # Maximum is 262144; 10^5 # Width is -100. The `flowCore::biexponentialTransform` fun ...
flowcore written 14 days ago by gene_bioconductor0 • updated 12 days ago by Jiang, Mike1.2k
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Comment: C: selecting multiple clusters from flowClust::tmixFilter
... Thanks, Mike. For a `tmixFilter` which returns multiple clusters, is there a way to turn an individual cluster (let's say cluster 4 out of k=6 clusters) as a gate? In the vignettes, I'm seeing gates accessed as "myGate+" or "myGate-", I haven't come across example code for something like "myGate ...
written 20 days ago by gene_bioconductor0
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selecting multiple clusters from flowClust::tmixFilter
... I'm trying to incorporate tmixFilter into a flowCore/flowWorkspace-based FACS data analysis. The primary problem I'm having is that I need to select multiple clusters the tmixFilter results and I can't find any examples a "flowCore"-y way of doing that. Below is what I have been doing, but it's ...
flowclust tmixfilter written 25 days ago by gene_bioconductor0 • updated 25 days ago by Jiang, Mike1.2k

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