User: Lucka387

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Lucka3870
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Posts by Lucka387

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Comment: C: CRISPRseek help to generate gRNAs for CRISPRi library
... Hi Julie, I adjusted my script based on your suggestions, and it worked well for my output but I am still unable to run on multiple cores. This is what I ran: ## Scenario 5: Target and off-target analysis for user specified gRNAs results <- offTargetAnalysis(inputFilePath = gRNAFilePath, enable. ...
written 12 days ago by Lucka3870
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Comment: C: CRISPRseek help to generate gRNAs for CRISPRi library
... Thank you very much for the prompt reply. I will rerun with the changed parameters as suggested. The other issue, which I mentioned, is that it is only using one core, which is odd considering the enable.multicore is set to true. Our system (just one server, not a cluster) has 144 available threads ...
written 15 days ago by Lucka3870
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CRISPRseek help to generate gRNAs for CRISPRi library
... I am currently in the process of designing a gRNA library for a CRISPRi screen. I would like to use the CRISPRseek script to identify the top 10 most efficient gRNAs per gene for my library. I ran a test on a small file (128 KB) using the script for Scenario #7: Quick gRNA finding with gRNA efficacy ...
crisprseek written 15 days ago by Lucka3870 • updated 15 days ago by Julie Zhu4.1k

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