User: William Kenworthy

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14 years, 5 months ago
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w**********@murdoch.edu.au

Posts by William Kenworthy

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Comment: C: technical replicates
... Unfortunately, they came to us too late and were forced by circumstances to complete the experiment as designed. Next time ... I am thinking a straight average of the replicate values for each gene may the best solution (not per probe). However, a significance value may show up a suspect gene (if ...
written 13.9 years ago by William Kenworthy70
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technical replicates
... Hi, I have just been passed a set of affy data that consists of 3 states, two technical replicates of each state (6 chips overall) 1. whats the best way (normalisations, algorithms) to leverage technical replicates? 2. how do you tell algorithms such as rma which are the replicates? (I presume the ...
affy written 13.9 years ago by William Kenworthy70 • updated 13.9 years ago by Naomi Altman6.0k
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Answer: A: RNA digestion plots
... RNA plots are something I have also been looking at, but all the datasets I have access to can be characterised as "good". Can anyone supply a (anonymous?) "cel" file of any persuasion that is known to have been used with degraded RNA? On larger arrays, I do see a slight slope, but not any "rollof ...
written 14.0 years ago by William Kenworthy70
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Answer: A: Status of binary CEL file support
... A similar question: is the old style .dat still available from the Affy GCOS software, or has that changed as well. BillK On Sat, 2003-11-15 at 00:30, Ben Bolstad wrote: > Hello all, > > There has been a number of recent requests for binary CEL file support. > I appreciate that many of ...
written 14.0 years ago by William Kenworthy70
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Answer: A: (no subject)
... 1. I have altered this sentence as it was confusing. Please check that it says what you intended. If not, please supply replacement text in this comment. Being able to align pairs *OF* very large of molecular sequences is an essential process for a range of comparative genomic studies 2. This phr ...
written 14.1 years ago by William Kenworthy70
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error when trying to load the affy library
... Hi, I just upgraded R to 1.8.0 and reloaded bioconductor and now get the following error when trying to load the affy library: > library(affy) Error in bindingIsLocked(m2, where) : not an environment Error in bindingIsLocked(m2, where) : not an environment Error in getClass(Class, where = topenv ...
affy written 14.1 years ago by William Kenworthy70 • updated 14.1 years ago by James W. MacDonald45k
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Microarray slide design and data analysis
... Hi, we have just bought in a few microarray slides and they have each gene characterised by only a single spot. This is not really desirable as I would like to see (ideally) a triplicate or at least a duplicate of spots distributed for each gene to allow some kind of quality check (variability) as ...
microarray affy written 14.5 years ago by William Kenworthy70

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