User: Manuela Di Russo

Reputation:
70
Status:
New User
Location:
Last seen:
7 years, 6 months ago
Joined:
8 years, 2 months ago
Email:
m**************@for.unipi.it

Posts by Manuela Di Russo

<prev • 7 results • page 1 of 1 • next >
0
votes
1
answer
991
views
1
answers
Comment: C: design matrix with technical and biologial replicates
... Dear James, if I have correctly interpreted, your suggestion is to fit the model to all probes on the array and then filter. But the approach usually adopted is based on filtering before fitting the linear model in order to reduce the number of hypotheses to test and increase the power. May I filter ...
written 7.5 years ago by Manuela Di Russo70
0
votes
1
answer
991
views
1
answers
Comment: C: design matrix with technical and biologial replicates
... Thank you James! I had already applied both methods you suggested but I wanted to see if there was a better way to handle this kind of experimental design. I have another question: I computed means of the duplicates arrays after preprocessing and filtering but before fitting the linear model, is thi ...
written 7.5 years ago by Manuela Di Russo70
0
votes
1
answer
991
views
1
answer
design matrix with technical and biologial replicates
... Dear list, I'm working with microarray expression data and I am using limma to detect differentially expressed genes. I have some questions about the design matrix and the handling of biological and technical replicates. The target file is: Sample_name sample_type sample_replicat ...
microarray limma written 7.5 years ago by Manuela Di Russo70 • updated 7.5 years ago by James W. MacDonald51k
0
votes
0
answers
529
views
0
answers
problems with oneChannelGUI
... Dear all, I have installed oneChannelGUI (CPU: Intel(R) Xeon(R) E5607 @2.27GHz (2 processors); RAM: 32 GB; OS: Windows 7, 64 bit) following the installation procedure. I load oneChannelGUI package and at the beginning it seems to work but when I try to select the targets file a black window opens ...
affylmgui onechannelgui assign written 7.6 years ago by Manuela Di Russo70
0
votes
1
answer
7.4k
views
1
answer
problems with "cannot allocate vector of size.."
... Dear all, I have some problems with the error "cannot allocate vector of size..." I am using the Bioc package "arrayQualityMetrics" to assess the quality of a microarray experiment saved in an Affybatch object (rawData) but I encountered the same problem with oneChannelGUI package, when I apply the ...
microarray gcrma written 7.6 years ago by Manuela Di Russo70 • updated 7.6 years ago by Wolfgang Huber13k
0
votes
1
answer
2.2k
views
1
answer
a problem in reading in cel files
... Dear all, I am learning to analyse Affymetrix microarray data but I have a problem in reading .cel files in. I downloaded from GEO the raw data provided as supplementary files (GSE12345_RAW.tar), than I have extracted the cel files in a directory which I have set as my working directory. Here is the ...
microarray written 7.7 years ago by Manuela Di Russo70 • updated 7.7 years ago by James F. Reid610
0
votes
1
answer
1.2k
views
1
answer
Some problems in combining ExpressionSet objects with the combine() function
... Hi all, I'm struggling with the meta-analysis of microarray data. In the user guide of the bioconductor package "MAMA" there is an exemple of meta- analysis in cancer microarray data concerning the comparison of expression profiles in MSI (microsatelite instable) and MSS (microsatelite stable) colo ...
microarray cancer colon limma written 8.2 years ago by Manuela Di Russo70 • updated 8.2 years ago by James W. MacDonald51k

Latest awards to Manuela Di Russo

Great Question 7.5 years ago, created a question with more than 5,000 views. For problems with "cannot allocate vector of size.."
Popular Question 7.5 years ago, created a question with more than 1,000 views. For a problem in reading in cel files
Popular Question 7.5 years ago, created a question with more than 1,000 views. For a problem in reading in cel files
Popular Question 7.5 years ago, created a question with more than 1,000 views. For problems with "cannot allocate vector of size.."

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 315 users visited in the last hour