User: Christopher Conley
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- 6 years ago
Posts by Christopher Conley
... The vignette of GOseq details in section 6.8 how to correct for total read count bias (per gene). The code used to calculate total read count bias incorporates raw coverage counts instead of normalized counts. When there are dramatic changes in library size, is it more appropriate to use the normal ...
... Having had personal communication with Dr. Evan Johnson on this very question, I am quoting his email response. > So my question is: Are there any reasons why using ComBat with RNA-seq data is not legit? Here is what Evan had to say: "For batch effects, if the sample sizes are large, say arou ...
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