User: aswathym

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aswathym0
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Joined:
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Posts by aswathym

<prev • 9 results • page 1 of 1 • next >
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Answer: A: Draft support for HTA 2.0 with oligo
... I don't know whether you have tried biocLite("pd.hta.2.0")  and then loaded using the command library. It worked for without any error I could load the cel files. ...
written 18 months ago by aswathym0
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Comment: C: miRNA expression analysis : annotation
... Here the annotaion file seleted is : pd.mirna.4.0  I dont know whether it is the correct file. I installed the particluar cdf file. whether it should be the annotation file. And how the miRNA annotation done ??  we can use getsymbol its for gene right I dont know exactly what should be done becau ...
written 2.7 years ago by aswathym0
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Answer: A: miRNA expression analysis : annotation
... > eset ExpressionSet (storageMode: lockedEnvironment) assayData: 36353 features, 4 samples    element names: exprs  protocolData   rowNames: C1_(miRNA-4_0).CEL C2_(miRNA-4_0).CEL G1_5_(miRNA-4_0).CEL     G1_8_(miRNA-4_0).CEL   varLabels: exprs dates   varMetadata: labelDescription channel phenoDa ...
written 2.7 years ago by aswathym0
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miRNA expression analysis : annotation
... Hii,                 I have few questions 1) I am doing miRNA expression analysis. the experiment was done using mouse model. I am finding difficulty to do annotation. The chip used is miRNA_4.0.(affymetrix). I extracted the expression dataset. what should be done next to annotate.  2) I got  &g ...
mirna annotation affymetrixchip written 2.8 years ago by aswathym0
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Comment: C: miRNA microarray analysis
... i did normalization. using the same procedure. Thank you so much ...
written 2.8 years ago by aswathym0
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Comment: C: miRNA microarray analysis
... Sir I solved that problem now its showing another eset.rma <- expresso(abatch, bgcorrect.method="rma", normalize.method="quantiles", pmcorrect.method="pmonly", summary.method="medianpolish") background correction: rma  normalization: quantiles  PM/MM correction : pmonly  expression values: medi ...
written 2.8 years ago by aswathym0
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Comment: C: miRNA microarray analysis
... sir I have the cdf file in the directory. Do I need to install in cmd ?? i have miRNA-4_0-st-v1 in my directory I read somewhere like that.   ...
written 2.8 years ago by aswathym0
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Comment: C: miRNA microarray analysis
... Thank you sir Still I tried all this. I couldn't understand what is the real problem.  make.cdf.package("mirna40cdf", version = packageDescription("makecdfenv", field = "Version"), species="", unlink=TRUE, compress=FALSE, package.path = pkgpath) Error in isCDFXDA(file.path(path.expand(cdf.path), f ...
written 2.8 years ago by aswathym0
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miRNA microarray analysis
... Hii,  I was working on this microRNA expression analysis. while doing I stuck by this error. Need to help to proceed further Thank you >library(affy) >data <- ReadAffy() >data AffyBatch object size of arrays=541x541 features (18 kb) cdf=miRNA-4_0 (??? affyids) number of samples=4 Er ...
microarray cdf written 2.8 years ago by aswathym0 • updated 2.8 years ago by Gordon Smyth32k

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