## User: Sushant Pawar

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sushant.pawar69
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#### Posts by Sushant Pawar

<prev • 23 results • page 2 of 3 • next >
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... Hi all , We want only the Gene Expression for the experiment without replicate, So i just want to confirm that is this code is wright or not . I am providing code below. And what should be the dispersion in "exactTest" . Please help me in that.   ============================================== l ...
written 4.0 years ago by Sushant Pawar0 • updated 4.0 years ago by Aaron Lun25k
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Comment: C: edgeR for with no Replicate
... Actually  i am new to this field , I have add this to gene. again i am getting some warning . so please help me . ============================= housekeeping=c("REG3A","GP2") > housekeeping=c("REG3A","GP2") > y0=estimateCommonDisp(y1[housekeeping,]) > y$common.dispersion=y0$common.dispe ...
written 4.0 years ago by Sushant Pawar0
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Comment: C: edgeR for with no Replicate
... but what type of Genes we should store in that variable ? ...
written 4.0 years ago by Sushant Pawar0
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Comment: C: edgeR for with no Replicate
... is it should be the vector or single gene name ? . give some example ...
written 4.0 years ago by Sushant Pawar0
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Comment: C: edgeR for with no Replicate
... is it should be the vector or single gene name . give some example   ...
written 4.0 years ago by Sushant Pawar0
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... Hi all, (edgeR Page no. 21,22 User Guide) I am running program of edgeR for without replicate . I am getting one one error so please help me in that . ==================================== > bcv=0.2 > library(edgeR) > counts=matrix(rnbinom(40,size=1/bcv^2,mu=10),20,2) > y=DGEList(cou ...
written 4.0 years ago by Sushant Pawar0 • updated 4.0 years ago by Yunshun Chen540
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... Hi all, Please provide me the commands of "edgeR" for differential express analysis without Replicate. ...
written 4.0 years ago by Sushant Pawar0 • updated 4.0 years ago by Aaron Lun25k
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... I have used this syntax for without Replicate by changing only sampleCondition command in DESeq2 . so, is it all wright  ========================================================== library(DESeq2) directory<-"/home/dgl/110_AmpliseqRNA/all_samples_with-replicates/1790_Cervical-Liver_with_LN_R2" s ...
written 4.0 years ago by Sushant Pawar0
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... Thank you guys , This is really helpful for me. ...
written 4.0 years ago by Sushant Pawar0
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... Hi all , I am running DESeq2 for Transcriptome analysis , Sometimes it is not possible to make replicate for each sample because it increases cost of analysis .So is it all right to simulate the replicate for case and control samples. for e.g - untreated (original counts of control)               ...
written 4.0 years ago by Sushant Pawar0

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