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DGE
•
reset
5
votes
4
replies
1.3k
views
Experiment design contrasts any time edgeR
DGE
rnaseq
RNA
edger
updated 7.0 years ago by
londonflavido
• 0 • written 7.0 years ago by
Pietro
▴ 40
5
votes
6
replies
1.6k
views
How to calculate dispersion DESeq2 - Step by Step
DGE
DESeq2
Differential
2.7 years ago
Zeke Smith
▴ 10
3
votes
7
replies
2.3k
views
How to determine the cause of no difference between treatments in rnaseq?
RNAseq
DGE
edger
updated 4.9 years ago by
Gordon Smyth
50k • written 4.9 years ago by
jdmontenegroc
• 0
0
votes
4
replies
2.7k
views
edgeR DGEList and design matrix
edger
DGE
updated 4.8 years ago by
Yunshun Chen
▴ 840 • written 4.8 years ago by
ctstackh
• 0
4
votes
8
replies
1.8k
views
Advice regarding RNAseq data with no replicates, best approach to accomplish fold-change
rnaseq
edger
DGE
no replicates
6.8 years ago
Stefany Solano
• 0
3
votes
8
replies
1.2k
views
Same contrasts returning different edgeR results
edger
limma
contrasts
DGE
updated 4.4 years ago by
James W. MacDonald
65k • written 4.4 years ago by
s.connell
• 0
5
votes
10
replies
1.3k
views
Correctly set contrast with DESeq2 for not full rank design matrix
DESeq2
DGE
contrast
updated 3.9 years ago by
Michael Love
42k • written 3.9 years ago by
llooll1
• 0
1
vote
2
replies
896
views
How to construct matrix and fit linear model for this microarray?
microarray
dge
matrix
makecontrasts
limma
updated 3.8 years ago by
Gordon Smyth
50k • written 3.8 years ago by
June.
▴ 10
7
votes
6
replies
482
views
Batch Effect Correction, Gene Filtering
DGE
limma
updated 8 weeks ago by
Gordon Smyth
50k • written 8 weeks ago by
Amit
• 0
0
votes
1
reply
477
views
How to do PFS after Voom + Limma DGE ?
RNASeqData
limma
voom
PFS
DGE
updated 10 months ago by
James W. MacDonald
65k • written 10 months ago by
manuelsokolov
• 0
12
votes
10
replies
6.5k
views
edgeR glmLRT with test="F"?
edger
dge
differential expression
updated 8.9 years ago by
Aaron Lun
★ 28k • written 8.9 years ago by
Ryan C. Thompson
★ 7.9k
0
votes
3
replies
1.7k
views
glmQLFTest, coef=2:4, decideTestDGE not working
edger
DGE
coef
updated 6.6 years ago by
Aaron Lun
★ 28k • written 6.6 years ago by
rebeccajane93
• 0
1
vote
5
replies
1.2k
views
edgeR or DESeq2 between duplicated chromosomes in the same individual
deseq2
edgeR
normalization
DGE
updated 4.6 years ago by
Gordon Smyth
50k • written 4.6 years ago by
grant.dejong
▴ 10
0
votes
2
replies
875
views
Longitudinal Time-Course microarray experiment with two time points
edge
DGE
microarray
updated 4.5 years ago by
Gordon Smyth
50k • written 4.5 years ago by
joanasabinopinto
• 0
0
votes
1
reply
1.6k
views
Doing differential expression using FPKM , samples don't have replication
dge
fpkm
rna-seq
updated 3.0 years ago by
Kevin Blighe
★ 3.9k • written 3.0 years ago by
lkianmehr
• 0
3
votes
4
replies
1.9k
views
DGE matrix annotation
DGE
DESeq2
AnnotationDbi
updated 5.6 years ago by
James W. MacDonald
65k • written 5.6 years ago by
maxininfa
• 0
1
vote
3
replies
667
views
Can CAMERA analyze just one particular pathway
pathway
ORA
limma
CAMERA
DGE
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
manuelsokolov
• 0
0
votes
3
replies
515
views
Deseq2, multifactor model design for comparing adult Mutant mouse versus pup Mutant mouse with normalization to the age difference of the Wildtype co…
DGE
DESeq2
7 months ago
ruba-mahmoud
• 0
1
vote
1
reply
724
views
DESeq2 - DGE in organoids for multiple group comparisons
personalome
organoids
DGE
DESeq2
updated 3.0 years ago by
Michael Love
42k • written 3.0 years ago by
S
▴ 10
0
votes
1
reply
837
views
DGE genes are not available in GSEA
dge
gsea
updated 17 months ago by
Guido Hooiveld
★ 4.0k • written 17 months ago by
Lawrence
• 0
0
votes
0
replies
906
views
quantifying piRNA and tRNA by Salmon
Salmon
DGE
5.5 years ago
lkianmehr
• 0
21 results • Page
1 of 1
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Answer: ERROR; return code from pthread_create() is 22
by
James W. MacDonald
65k
Seems OK to me. ``` > dat <- ReadAffy() > dat AffyBatch object size of arrays=744x744 features (20 kb) cdf=HG-U219 (49386 affyids) …
Answer: LRT, more than two conditions and pairwise comparisons
by
James W. MacDonald
65k
Unless you think the different lines or tissues will have very different within-group variances, you should just analyze together and make …
Answer: How to remove batch effect to combine RNAseq data from 2 different experiments?
by
James W. MacDonald
65k
It appears that batch is orthogonal to the treatment, so you can just fit a batch effect in your model.
Answer: Control group between 2 batches have different expression in RNAseq data
by
James W. MacDonald
65k
Since batch is orthogonal to the treatment, you just add a batch factor to your model.
Answer: Subsetting RNAseq data | voomaByGroup()
by
Anna
• 0
Hi Gordon. Thank you so much. Your reply is extremely helpful, and I feel more confident moving forward with the analysis. Also, very inte…
Votes
Answer: Subsetting RNAseq data | voomaByGroup()
biomaRt not working - lazy table error
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
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