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Bile acid analysis with limpa
limpa
MassSpectrometry
MassSpectrometryData
6 hours ago
DN
• 0
0
votes
6
replies
174
views
Making a Full Rank Model for Allele-Specific Expression with DESeq2
DESeq2
updated 4 hours ago by
Gordon Smyth
53k • written 1 day ago by
chp265
• 0
0
votes
0
replies
22
views
Changing multiple display parameters of a specific exon in GeneRegionTrack
Gviz
1 day ago
Xenia
• 0
0
votes
0
replies
19
views
Justify title track when rotation = 0
Gviz
gviz
1 day ago
Xenia
• 0
0
votes
1
reply
139
views
Are individual-mouse-based statistics possible in CellChat?
Bioconductor
CellChat
updated 1 day ago by
ATpoint
★ 5.0k • written 8 days ago by
Omer
• 0
0
votes
6
replies
530
views
BiocParallel (and DESeq2) - wrong args for environment subassignment
BiocParallel
DESeq2
updated 2 days ago by
Martin Morgan
25k • written 7 weeks ago by
Benjamin
• 0
0
votes
1
reply
204
views
DESeq2 - how to shrink LFC for pairwise comparisons having multiple groups
shrinkage
DESeq2
contrast
updated 1 day ago by
ATpoint
★ 5.0k • written 5 days ago by
ja569116
• 0
1
vote
3
replies
204
views
limpa analysis advice
limpa
updated 3 days ago by
Gordon Smyth
53k • written 5 days ago by
SamGG
▴ 360
0
votes
0
replies
38
views
News:
The BioC2026 Sticker Design Contest is Open!
Bioconductor
Conference
6 days ago
laurah ondari
• 0
4
votes
2
replies
213
views
When to use edgeR or limma
edgeR
limma
updated 5 days ago by
Gordon Smyth
53k • written 8 days ago by
abzhang97
• 0
1
vote
3
replies
178
views
Incosistencies in buildSNNGraph() function
scran
seurat
written 11 days ago by
Lucas
• 0
0
votes
2
replies
728
views
A problem occurs when running the monocle2
monocle
written 4 months ago by
yuheng_yang
• 0
0
votes
7
replies
358
views
limpa-blank normalization and Spectronaut's PTM stoichiometry
limpa
updated 9 hours ago by
Gordon Smyth
53k • written 7 days ago by
emily.wolfrum
• 0
0
votes
1
reply
79
views
Questions about bacon QC and correction on EWAS results
bacon
8 days ago
sup0903
• 0
3
votes
4
replies
243
views
Question about the filterByExpr function inputs in edgeR
RNASeq
filter
design
edgeR
model.matrix
8 days ago • updated 7 days ago
mohammedtoufiq91
▴ 10
0
votes
1
reply
99
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Development of MSstat workflow to analyze DIA time series data
MSstats
updated 8 days ago by
Gordon Smyth
53k • written 8 days ago by
sithurandi
• 0
0
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0
replies
102
views
MSstatsPTM-SpectronauttoMSstatsPTMFormat-failing with TAIR10 Locus Tags: Issue with UniProt-centric protein ID parsing
Bioconductor
MSstatsPTM
9 days ago
srkingwu
• 0
8
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8
replies
2.9k
views
Confounded design, batch effect correction (edgeR)
rnaseq
edger
differential gene expression
batch effect
written 7.4 years ago by
Laura
▴ 20
0
votes
0
replies
59
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Junction CPM via Snapcount and Recount3
snapcount
recount3
9 days ago
henry.tietz
• 0
3
votes
4
replies
356
views
Clarification on counting in Rsubread (featureCounts)
featureCounts
Rsubread
updated 8 days ago by
Gordon Smyth
53k • written 5 weeks ago by
Luca
• 0
0
votes
0
replies
55
views
News:
Online RNA-seq analysis course 26-29th Janury
EnhancedVolcano
GSEABase
pheatmap
Workshop
DESeq2
9 days ago
Frances Turner
▴ 20
0
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0
replies
56
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How to convert .raw data for use in xcms
xcms
10 days ago
Monica
• 0
0
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0
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116
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Job:
Open Positions for Doctoral Researchers in Molecular Medicine
JobPosting
10 days ago
rubina.tabassum
• 0
1
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0
replies
93
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News:
Bioconductor Maintainer Application
maintainer
policy
Bioconductor
Devel
devel
12 days ago
shepherl
4.3k
0
votes
1
reply
461
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News:
GSEA in R/Bioconductor course
GSEABenchmarkeR
pairedGSEA
GeneSetEnrichment
written 10 months ago by
info
▴ 10
0
votes
1
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336
views
News:
Announcing a New Journal - Bioinformatics Methods and Applications - Now Open for Submissions
Bioconductor
written 6 months ago by
Wei Shi
★ 3.6k
0
votes
2
replies
2.5k
views
ChAMP pipeline error (champ.import)
software error
written 6.8 years ago by
mhebba
• 0
0
votes
2
replies
228
views
Combining two proteomics datasets with limpa
limpa
ProteomicsWorkflow
limma
14 days ago • updated 8 hours ago
Andrew Pattison
• 0
0
votes
0
replies
128
views
Job:
Jobs at Ensembl
ensembldb
16 days ago
Aleena_Ensembl
▴ 30
0
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0
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11
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Spam:
Is there a way to map Excel fields to VCF contacts for accurate conversion?
ComputationalChemistry
Technology
Software
Website
updated 16 days ago by
shepherl
4.3k • written 16 days ago by
witebir963
0
0
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0
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80
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Job:
Job:Computational Biologist position at the University of Alabama at Birmingham
hiring
RNASeq
JobPosting
SingleCell
SpatialData
16 days ago
lianov
• 0
2
votes
3
replies
218
views
please provide a BibTeX citation for "Advanced Single-Cell Analysis with Bioconductor"
Bioconductor
written 17 days ago by
Victor
▴ 10
2
votes
4
replies
229
views
Converting Probe IDs to HGNC symbols
Annotation
biomaRt
GEOquery
written 19 days ago by
Manav
▴ 10
0
votes
3
replies
274
views
How to do differential expresion across time serise with edgeR?
edgeR
timecoursedata
updated 18 days ago by
Gordon Smyth
53k • written 19 days ago by
petergrahamclark
▴ 10
2
votes
3
replies
445
views
Guitar R package requires update due to dependency GenomicFeatures
GenomicFeatures
Guitar
txdbmaker
GenomicRanges
written 23 days ago by
ferran.llobet.cabau
▴ 10
0
votes
2
replies
177
views
ATACseq rudimentary questions
ATACSeq
DiffBind
25 days ago • updated 19 days ago
4747
• 0
1
vote
1
reply
217
views
How to install packages such as TCGAbiolinks, SumarizedExperiment?
TCGAbiolinks
SummarizedExperiment
updated 21 days ago by
Gordon Smyth
53k • written 21 days ago by
NewRUser
• 0
0
votes
4
replies
5.6k
views
blockwiseModule crashes in the end and doesn't generate modules
WGCNA
written 2.8 years ago by
mauricio.microbiol
• 0
4
votes
2
replies
392
views
Limma-voom sample weights and impact on DEG numbers
limma
4 weeks ago • updated 17 days ago
A.Barden
▴ 20
0
votes
1
reply
267
views
wgcna module preseravtion taking too long?
WGCNA
updated 29 days ago by
ATpoint
★ 5.0k • written 5 weeks ago by
lamia11me
• 0
0
votes
1
reply
353
views
Question about RE-agnostic mode for intact Hi-C and Micro-C normalization(HiCDCPlus)
HiC
HiCDCPlus
written 6 weeks ago by
Chaoability
• 0
0
votes
3
replies
329
views
DESeq2 - how many surrogate variables from svaseq, use of lfcshrink
sva
lfcshrink
DESeq2
4 weeks ago • updated 5 days ago
ja569116
• 0
0
votes
5
replies
2.9k
views
Ensembl site unresponsive via biomaRt
biomaRt
written 10 months ago by
hebda
• 0
0
votes
3
replies
288
views
segfault in edgeR glmQLFit
edgeR
updated 4 weeks ago by
Gordon Smyth
53k • written 4 weeks ago by
Mark
• 0
0
votes
4
replies
594
views
How does contrasts.fit do in limma to extract comparisons with a model having interaction terms?
limma
updated 16 days ago by
jtsy6383
• 0 • written 4 weeks ago by
Durian
• 0
0
votes
1
reply
263
views
News:
Opportunity to host EuroBioC2026 Preconference workshops!
Conference
Workshop
Bioconductor
written 5 weeks ago by
laurah ondari
• 0
0
votes
1
reply
157
views
NUPOP usage
TOAST
updated 5 weeks ago by
shepherl
4.3k • written 5 weeks ago by
Mohammad
• 0
1
vote
2
replies
365
views
News:
Important: Bioconductor Release 3.22 Delayed
release
written 12 weeks ago by
shepherl
4.3k
0
votes
1
reply
238
views
PTM analysis in LIMPA
limpa
updated 6 days ago by
Gordon Smyth
53k • written 7 weeks ago by
Diego FGR
• 0
1
vote
1
reply
278
views
News:
Upcoming Ensembl API and data access changes - new blog post available
ensembl
mart
biomaRt
ensembldb
written 7 weeks ago by
Ensembl_Jorge
▴ 10
46,730 results • Page
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Comment: Combining two proteomics datasets with limpa
by
Andrew Pattison
• 0
Thanks heaps Gordon. I went with option 1 and no normalisation and all seems to have worked well. Cheers, Andrew
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
Flagging protein groups as likely contaminants in this way will be fine for a limpa analysis. The `Cont_` proteins can optionally stay in f…
Answer: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Gordon Smyth
53k
Aaron Lun was a PhD student in my Lab back in 2015, when he posted the answers about allele-specific expression that you and Mike Love have…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Michael Love
43k
You just need the mouse identifier (controls baseline) and the group specific allelic effect.
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Julia Broadbent
• 0
Hi Emily, Just sharing a resource for handling contaminants - we use the methods described in [Frankenfield et al. (2022)](https://pubs.acs…
Votes
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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