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Showing :
limma
•
reset
1
vote
6
replies
2.7k
views
Can I use EdgeR log2(cpm) instead of voom log2(cpm) to be analyzed as microarray data?
limma
edger
timecourse
without replicate
voom
written 7.5 years ago by
Camila Orellana
▴ 10
0
votes
6
replies
3.1k
views
limma, analysis on subset of data gives completely different results
limma
limma
updated 6 weeks ago by
Gordon Smyth
50k • written 9.8 years ago by
Arvid Sondén
▴ 40
2
votes
8
replies
568
views
Microarray Limma Model fitting and DEG
limma
DGEanalysis
updated 7 weeks ago by
Gordon Smyth
50k • written 7 weeks ago by
Amit
• 0
1
vote
6
replies
954
views
Limma dispersed distribution of log fold change
limma
written 8 months ago by
Erin Yu
• 0
9
votes
5
replies
5.1k
views
Differential expression analysis: Permutation Test
deseq2
limma
edger
permutation
updated 5 months ago by
Gordon Smyth
50k • written 8.2 years ago by
Radek
▴ 90
15
votes
17
replies
1.3k
views
Why p value inconsistent when using different number of pathway score?
limma
4 weeks ago • updated 18 days ago
Chris
▴ 10
8
votes
6
replies
509
views
DGE analysis with LIMMA
limma
updated 11 weeks ago by
Gordon Smyth
50k • written 11 weeks ago by
Amit
• 0
0
votes
4
replies
396
views
limma with interaction term
R
biostatistics
limma
Epigenetics
3 months ago • updated 12 weeks ago
Jitendra
▴ 10
1
vote
2
replies
81
views
Subsetting RNAseq data | voomaByGroup()
limma
2 days ago • updated 1 hour ago
Anna
• 0
6
votes
5
replies
6.4k
views
Using Limma R package For Proteomics differential expression
limma
ProteomicsWorkflow
updated 8 months ago by
Gordon Smyth
50k • written 2.5 years ago by
David Lyon
▴ 340
3
votes
11
replies
1.4k
views
SD filtering before DEG analysis with limma : possible or not ?
filtering
STD
DEG
limma
updated 11 months ago by
Gordon Smyth
50k • written 11 months ago by
alexia.brunel
• 0
4
votes
2
replies
1.3k
views
Gene set analysis: GSVA, Z-Score, and fold-changes
GSVA
RNASeq
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
Jonathan
▴ 10
3
votes
2
replies
465
views
For limma, if I leave coef=NULL, which test is the P value for?
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
S
• 0
1
vote
2
replies
407
views
limma Intercept vs No-intercept models completely changing DMR results?
limma
DMRcate
MethylationArray
EPICV2
updated 16 days ago by
Gordon Smyth
50k • written 16 days ago by
Kim
• 0
1
vote
2
replies
475
views
Assess fit of variance prior
limma
updated 5 months ago by
Gordon Smyth
50k • written 5 months ago by
Frederik Ziebell
▴ 20
1
vote
3
replies
1.9k
views
Making MDS plot
plotMDS
limma
updated 11 months ago by
Gordon Smyth
50k • written 11 months ago by
liujy4
• 0
1
vote
2
replies
1.2k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 12 months ago by
informatician
• 0
0
votes
3
replies
484
views
'Subtracting' counts on basis of Negative control media and water samples
limma
edgeR
RNASeq
DESeq2
6 months ago • updated 5 months ago
Karthik
• 0
0
votes
2
replies
651
views
Fluidigm Data Batch Correction
sva
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
Khushbu
• 0
1
vote
3
replies
369
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 5 weeks ago by
Gordon Smyth
50k • written 5 weeks ago by
Simran
• 0
0
votes
3
replies
1.5k
views
Limma, eBayes different sample size of groups and adjustment
limma
limma
updated 19 days ago by
XW
• 0 • written 13.1 years ago by
Babara Mueller
▴ 10
1
vote
7
replies
884
views
DuplicateCorrelation() block by duplicate sample or by cell line ?
variancePartition
RNASeqData
limma
BulkRNAseq
RNASeq
updated 5 months ago by
Gordon Smyth
50k • written 7 months ago by
Gimena
• 0
0
votes
5
replies
975
views
minfi, limma, adjusting for cell counts - integrating the resoults of estimateCellCounts into the linear regression model
minfi
limma
7 months ago
dorota.komar
• 0
11
votes
4
replies
1.9k
views
LIMMA dupcor() function time issue.
limma
microarray
block
updated 7 months ago by
Gordon Smyth
50k • written 5.3 years ago by
tleona3
• 0
1
vote
3
replies
443
views
Cross-species analysis - how to compute the mid parent value?
DESeq2
limma
edgeR
DifferentialExpression
written 3 months ago by
pl23
• 0
2
votes
5
replies
1.4k
views
removing the batch effect for vst counts and defining the batches in removeBatchEffect function
DESeq2
BatchEffect
limma
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
Sara
• 0
5
votes
2
replies
607
views
Filter genes by expression for voomLmFit
DEG
edgeR
limma
voom
filterByExpr
updated 5 months ago by
Gordon Smyth
50k • written 5 months ago by
annikagable
• 0
0
votes
1
reply
163
views
Gene-by-sample covariates in limma
Metabolomics
limma
updated 17 days ago by
Gordon Smyth
50k • written 17 days ago by
c53aba27
• 0
4
votes
7
replies
2.1k
views
should statmod be a dependency of limma and not a suggests?
limma
updated 9 months ago by
Gordon Smyth
50k • written 8.0 years ago by
nathan
• 0
0
votes
2
replies
322
views
limma model matrix questions
limmaGUI
limma
pairedsamples
3 months ago
whyw6948
• 0
5
votes
3
replies
317
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 10 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Shaimaa Gamal
• 0
5
votes
8
replies
8.9k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 27 days ago by
hortense96
• 0 • written 7.8 years ago by
dmcleanuk
▴ 30
1
vote
3
replies
667
views
Can CAMERA analyze just one particular pathway
pathway
ORA
limma
CAMERA
DGE
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
manuelsokolov
• 0
2
votes
1
reply
314
views
removeBatchEffect with non-linear model fit
limma
DESeq2
updated 5 months ago by
Gordon Smyth
50k • written 5 months ago by
georgii.vdovin
• 0
5
votes
6
replies
3.8k
views
Differential protein/biomarker expression using limma: is that possible?
Proteomics
NPX
limma
updated 3 months ago by
briankleiboeker
• 0 • written 3.2 years ago by
r.i.s.alnuwaysir
▴ 10
0
votes
9
replies
593
views
limma fo continous variables
limma
DEGseq
updated 12 weeks ago by
James W. MacDonald
65k • written 12 weeks ago by
mia
• 0
0
votes
3
replies
431
views
Agilent Microarray Gene Oncology analysis gone wrong
AgilentChip
limma
goana
updated 5 months ago by
SamGG
▴ 350 • written 5 months ago by
samagbeyimodupe
• 0
1
vote
1
reply
374
views
Further analysis after limma regression output
statistics
Epigenetics
limma
updated 8 months ago by
James W. MacDonald
65k • written 8 months ago by
Jitendra
▴ 10
0
votes
2
replies
1.4k
views
Calculation of t-tests for factorial design
Microarray
limma
Microarray
limma
written 13.7 years ago by
Pascal Gellert
▴ 80
0
votes
1
reply
515
views
Is it possible to combine count gene expression data coming from one sequencing technology (RNA seq) with intensity gene expression data coming from …
DESeq2
RNASeqData
edgeR
limma
DifferentialExpression
updated 8 months ago by
ATpoint
★ 4.1k • written 8 months ago by
Sep
• 0
5
votes
5
replies
827
views
limma output for epic array methylation data
linearregression
epicarray
MethylationArray
limma
9 months ago
Jitendra
▴ 10
2
votes
4
replies
646
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom
RNASeq
limma
6 months ago
Tyler Sagendorf
▴ 10
0
votes
2
replies
304
views
Pairwise analysis of DESeq result and limma result
DESeq2
limma
3 months ago
Lily Cheang
▴ 10
5
votes
9
replies
980
views
Light difference when using coef vs omit a group when compare 3 groups.
limma
5 weeks ago • updated 4 weeks ago
Chris
▴ 10
0
votes
6
replies
812
views
Questionable limma results for differential expression on RNA-seq
RNASeqData
DifferentialExpression
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
gld-bioinf
• 0
5
votes
2
replies
703
views
PhD student
TMM
limma
10 months ago
Nisha
• 0
1
vote
2
replies
615
views
Extracting fitted expression matrix from Limma
limma
11 months ago
sarah.kelliher
• 0
1
vote
4
replies
748
views
Pairwise differential expression using limma
limma
10 months ago
Melissa
• 0
0
votes
6
replies
872
views
Help with model.matrix and creating the right contrast matrix for limma
limma
8 months ago • updated 5 months ago
rober204
• 0
1
vote
12
replies
2.5k
views
Is there a need or general principle/rule to assess whether the data types/distribution etc is suitable for limma R package to derive differential fe…
limma
highthroughputassays
R
updated 3 months ago by
Mary
• 0 • written 2.6 years ago by
Ming
▴ 380
50 results • Page
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Answer: ERROR; return code from pthread_create() is 22
by
James W. MacDonald
65k
Seems OK to me. ``` > dat <- ReadAffy() > dat AffyBatch object size of arrays=744x744 features (20 kb) cdf=HG-U219 (49386 affyids) …
Answer: LRT, more than two conditions and pairwise comparisons
by
James W. MacDonald
65k
Unless you think the different lines or tissues will have very different within-group variances, you should just analyze together and make …
Answer: How to remove batch effect to combine RNAseq data from 2 different experiments?
by
James W. MacDonald
65k
It appears that batch is orthogonal to the treatment, so you can just fit a batch effect in your model.
Answer: Control group between 2 batches have different expression in RNAseq data
by
James W. MacDonald
65k
Since batch is orthogonal to the treatment, you just add a batch factor to your model.
Answer: Subsetting RNAseq data | voomaByGroup()
by
Anna
• 0
Hi Gordon. Thank you so much. Your reply is extremely helpful, and I feel more confident moving forward with the analysis. Also, very inte…
Votes
Answer: Subsetting RNAseq data | voomaByGroup()
biomaRt not working - lazy table error
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
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