Hello Sir/Madam,

Thank you for your attention.

I am Lei, a graduate student who try to use your flowcore package to open .FCS files. Can I ask you a question? Thanks!

I set up flowcore in R in Linux. Then I typed the example code from your pdf "data structures package for flow cytometry data"

> file.name <- system.file("extdata","0877408774.B08", 
> package="flowCore") x <- read.FCSfile.name, transformation=FALSE) 
> summary(x)

The above codes ran quite well in my Linux. And then I pasted my own .FCS file "0001.FCS" into extdata folder, and tried to run it. 

> file.name <- system.file("extdata","0001.FCS", package="flowCore")
> x <- read.FCSfile.name, transformation=FALSE)
Warning message:
The file contains 1 additional data segment. The default is to read the first segment only.
Please consider setting the 'dataset' argument. 

However, the above warning message showed up. Can you please give me a suggestion? Thank!

Best,

Lei