Hi,
I want to use sleuth on the data salmon returned. In the first step, because sleuth needs actually kallisto, I used wasabi to generate the abundance.h5 file. However, after using

so <- sleuth_prep(s2c, ~ condition, num_cores = 1)

(s2c looks like that: )

sample condition
1 sample1         D
2 sample2         D
3 sample3         D
4 sample4         C
5 sample5         C
6 sample6         C
                                                                                   path
1 path/to/folder/sample1
2 path/to/folder/sample2

2 path/to/folder/sample3

2 path/to/folder/sample4

2 path/to/folder/sample5

2 path/to/folder/sample6

I get the following error:

reading in kallisto results
dropping unused factor levels
......
normalizing est_counts
117728 targets passed the filter
normalizing tpm
merging in metadata
summarizing bootstraps
.Error in process_bootstrap(i, samp_name, kal_path, num_transcripts, est_counts_sf[[i]],  :
  File h5 has no bootstraps.Please generate bootstraps using "kallisto quant -b".File Y:/AG_Huebner/Eric/tmp_for_jobs/seq_polyA_trim/hs_li_001_D_mR_tr_clean.fastq.gz_quant/abundance.h5 has no bootstraps.Please generate bootstraps using "kallisto quant -b".

My session Info:

Session info --------------------------------------------------------------------------------------------
 setting  value                       
 version  R version 3.4.2 (2017-09-28)
 system   i386, mingw32               
 ui       RStudio (1.1.383)           
 language (EN)                        
 collate  English_United States.1252  
 tz       Europe/Berlin               
 date     2017-11-07