code are below.
Thank you!
library(limma) library(oligo) library(pd.clariom.s.human) celFiles <- list.celfiles('.', full.names=TRUE) ab<- read.celfiles(celFiles) eset<- oligo::rma(ab) library("affycoretools") eset <- annotateEset(eset, annotation(eset)) Error: There appears to be a mismatch between the ExpressionSet and the annotation data. Please ensure that the summarization level for the ExpressionSet and the 'type' argument are the same. See ?annotateEset for more information on the type argument. eset ExpressionSet (storageMode: lockedEnvironment) assayData: 27189 features, 37 samples element names: exprs protocolData rowNames: Pa25N1_101817_(Clariom_S_Human).CEL Pa25T1_101817_(Clariom_S_Human).CEL ... Pa37T2_101817_(Clariom_S_Human).CEL (37 total) varLabels: exprs dates varMetadata: labelDescription channel phenoData rowNames: Pa25N1_101817_(Clariom_S_Human).CEL Pa25T1_101817_(Clariom_S_Human).CEL ... Pa37T2_101817_(Clariom_S_Human).CEL (37 total) varLabels: index varMetadata: labelDescription channel featureData: none experimentData: use 'experimentData(object)' Annotation: pd.clariom.s.human > sessionInfo() R version 3.4.2 (2017-09-28) Platform: x86_64-apple-darwin15.6.0 (64-bit) Running under: OS X El Capitan 10.11.5 Matrix products: default BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib LAPACK: /Library/Frameworks/R.framework/Versions/3.4/Resources/lib/libRlapack.dylib locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages: [1] grid stats4 parallel stats graphics grDevices utils datasets methods base other attached packages: [1] affycoretools_1.48.0 ggrepel_0.7.0 [3] stringr_1.2.0 pd.clariom.s.human_3.14.1 [5] DBI_0.7 oligo_1.40.2 [7] oligoClasses_1.38.0 RSQLite_2.0 [9] Biostrings_2.44.2 XVector_0.16.0 [11] limma_3.32.10 ComplexHeatmap_1.14.0 [13] bindrcpp_0.2 rtracklayer_1.36.5 [15] dplyr_0.7.4 purrr_0.2.3 [17] readr_1.1.1 tidyr_0.7.1 [19] tibble_1.3.4 ggplot2_2.2.1 [21] tidyverse_1.1.1 org.Hs.eg.db_3.4.1 [23] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2 GenomicFeatures_1.28.5 [25] AnnotationDbi_1.38.2 Biobase_2.36.2 [27] GenomicRanges_1.28.6 GenomeInfoDb_1.12.3 [29] IRanges_2.10.5 S4Vectors_0.14.7 [31] BiocGenerics_0.22.1 here_0.1 loaded via a namespace (and not attached): [1] R.utils_2.6.0 htmlwidgets_0.9 trimcluster_0.1-2 [4] BiocParallel_1.10.1 munsell_0.4.3 codetools_0.2-15 [7] preprocessCore_1.38.1 colorspace_1.3-2 BiocInstaller_1.26.1 [10] OrganismDbi_1.18.1 Category_2.42.1 knitr_1.17 [13] robustbase_0.92-7 labeling_0.3 GenomeInfoDbData_0.99.0 [16] hwriter_1.3.2 mnormt_1.5-5 bit64_0.9-7 [19] rprojroot_1.2 biovizBase_1.24.0 diptest_0.75-7 [22] affxparser_1.48.0 R6_2.2.2 locfit_1.5-9.1 [25] AnnotationFilter_1.0.0 flexmix_2.3-14 bitops_1.0-6 [28] reshape_0.8.7 DelayedArray_0.2.7 assertthat_0.2.0 [31] scales_0.5.0 nnet_7.3-12 gtable_0.2.0 [34] affy_1.54.0 ggbio_1.24.1 ensembldb_2.0.4 [37] rlang_0.1.2 genefilter_1.58.1 GlobalOptions_0.0.12 [40] splines_3.4.2 lazyeval_0.2.0 acepack_1.4.1 [43] dichromat_2.0-0 broom_0.4.2 checkmate_1.8.4 [46] yaml_2.1.14 reshape2_1.4.2 modelr_0.1.1 [49] backports_1.1.1 httpuv_1.3.5 Hmisc_4.0-3 [52] RBGL_1.52.0 tools_3.4.2 psych_1.7.8 [55] gplots_3.0.1 affyio_1.46.0 ff_2.2-13 [58] RColorBrewer_1.1-2 Rcpp_0.12.13 plyr_1.8.4 [61] base64enc_0.1-3 zlibbioc_1.22.0 RCurl_1.95-4.8 [64] rpart_4.1-11 GetoptLong_0.1.6 viridis_0.4.0 [67] SummarizedExperiment_1.6.5 haven_1.1.0 cluster_2.0.6 [70] magrittr_1.5 data.table_1.10.4-3 circlize_0.4.1 [73] mvtnorm_1.0-6 whisker_0.3-2 ProtGenerics_1.8.0 [76] matrixStats_0.52.2 hms_0.3 mime_0.5 [79] xtable_1.8-2 XML_3.98-1.9 gcrma_2.48.0 [82] mclust_5.3 readxl_1.0.0 gridExtra_2.3 [85] shape_1.4.3 compiler_3.4.2 biomaRt_2.32.1 [88] KernSmooth_2.23-15 R.oo_1.21.0 ReportingTools_2.16.0 [91] htmltools_0.3.6 GOstats_2.42.0 Formula_1.2-2 [94] geneplotter_1.54.0 lubridate_1.6.0 MASS_7.3-47 [97] fpc_2.1-10 Matrix_1.2-11 gdata_2.18.0 [100] R.methodsS3_1.7.1 bindr_0.1 forcats_0.2.0 [103] pkgconfig_2.0.1 GenomicAlignments_1.12.2 foreign_0.8-69 [106] xml2_1.1.1 foreach_1.4.3 annotate_1.54.0 [109] AnnotationForge_1.18.2 rvest_0.3.2 VariantAnnotation_1.22.3 [112] digest_0.6.12 graph_1.54.0 cellranger_1.1.0 [115] htmlTable_1.9 dendextend_1.5.2 edgeR_3.18.1 [118] GSEABase_1.38.2 curl_3.0 kernlab_0.9-25 [121] gtools_3.5.0 shiny_1.0.5 Rsamtools_1.28.0 [124] modeltools_0.2-21 rjson_0.2.15 nlme_3.1-131 [127] jsonlite_1.5 PFAM.db_3.4.1 viridisLite_0.2.0 [130] BSgenome_1.44.2 lattice_0.20-35 GGally_1.3.2 [133] httr_1.3.1 DEoptimR_1.0-8 survival_2.41-3 [136] GO.db_3.4.1 interactiveDisplayBase_1.14.0 glue_1.1.1 [139] prabclus_2.2-6 iterators_1.0.8 bit_1.1-12 [142] class_7.3-14 stringi_1.1.5 blob_1.1.0 [145] DESeq2_1.16.1 AnnotationHub_2.8.3 caTools_1.17.1 [148] latticeExtra_0.6-28 memoise_1.1.0
Thanks James. I upgraded R to the current. and somehow the old bioc packages are still linked to the new R version...
The other way indeed worked.
Best,
Ming