HTqPCR for Fluidigm Results
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rhonce • 0
@rhonce-15053
Last seen 6.8 years ago

Hi! I just ran a 96.96 on the biomark Fluidigm platform and I'm trying to analyze in R. I've been using a guide from Heidi Dvinge - "HTqPCR - high-throughput qPCR analysis in R and Bioconductor" but I've run into issues just importing my data. Each time I get the error "could not find function 'readCtdata'

I've installed Bioconductor and the HTqPCR package but am I missing something?

> # Locate example data
> exPath <- system.file("fluidigm", package="HTqPCR")
> exFiles <- "fluidigm"
> # Create qPCRset object
> raw    <- readCtData("fluidigm.csv", path="/Users/Desktop", n.features=96, n.data=96, format="BioMark")

Warning message:
package ‘readctdata’ is not available (for R version 3.4.1) 

Any help is appreciated! Thanks.

htqpcr fluidigm • 953 views
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