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thomasjenner333
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@thomasjenner333-15064
Last seen 6.8 years ago
Hi,
I'm trying to do pathway analysis for DE porcine genes generated using DESeq2.
(A) I used package "clusterprofiler" to convert gene symbols to enterzids using the following code:
gg = bitr(m1, fromType="SYMBOL", toType="ENTREZID", OrgDb="org.Ss.eg.db") geneSS <- as.vector(gg$ENTREZID)
(B) Then I implemented 'enrichPathway' function to obtain enriched pathways. The following is the code I used, but it failed to generate the enriched pathways.
rpa <- enrichPathway(gene=geneSS, pvalueCutoff=0.05, readable=T, organism="pig") head(as.data.frame(rpa)) [1] ID Description GeneRatio BgRatio pvalue p.adjust [7] qvalue Count <0 rows> (or 0-length row.names)
I was able to get a list of KEGG pathways using "erichKEGG". But, I couldn't do the same with ReactomePA. Can anyone help solve this? Thanks in advance.
Hi Yu!
Thank you for responding. I tried what you've suggested, and got a list of pathways. Could you please explain why the previous parameters didn't work? Thanks once again.
maybe you would find the blog post useful.
Thanks you.
Likely because Guangchuang did not specify any significance cutoff (he set the cutoffs for both p-value and q-value to 1, i.e. no significance filtering), and you set the p-value cutoff at 0.05 (which is apparently too strict for your experiment).