DiffBind different read counts depending on dba.count scores specified
0
0
Entering edit mode
Lauma R ▴ 10
@lauma-r-14513
Last seen 6.2 years ago
Cambridge

After closer inspection I noticed that Reads and RPKM values for the same samples in dba.counts$peaks changes depending which score metric I use. I was not quite sure if it something that should be expected, If that is the case, than why?

My code is:

dba.count_NON_INF.Reads <- dba.count( peak.test.NONINF, peaks= GRanges_NonINF, bRemoveDuplicates = FALSE, score = DBA_SCORE_READS )

dba.count_NON_INF.Reads$peaks
[[1]]
             
 
 
 
Chr     Start       End Score         RPKM Reads cRPKM cReads
1chr1     10014     10397    14   11.2965190    14     1      1
2chr1    180723    180910     6    9.9157375     6     1      1
3chr1    181437    181568     2    4.7181753     2     1      1
4chr1    629102    629953  2930 1064.0289316  2930     1      1
5 chr1    633374    634042  3603 1666.8755497  3603     1      1
6chr1    778350    778907    72   39.9477826    72     1      1
7chr1    827085    827736    30   14.2414970    30     1      1
8chr1    869749    870148    13   10.0689882    13     1      1
 
 
 
 
 
 
 
 
 
 
dba.count_NON_INF.RPKM <- dba.count(peak.test.NONINF, peaks= GRanges_NonINF, bRemoveDuplicates = FALSE, score = DBA_SCORE_RPKM )

dba.count_NON_INF.RPKM$peaks
[[1]]
               
 
 

 
Chr     Start       End        Score         RPKM Reads cRPKM cReads
1chr1     10014     10397   11.2965190   11.2965190    14     1      1
2chr1    180723    180910    8.2631146    8.2631146     5     1      1
3chr1    181437    181568    7.0772630    7.0772630     3     1      1
4chr1    629102    629953 1147.5533870 1147.5533870  3160     1      1
5chr1    633374    634042 1848.2286486 1848.2286486  3995     1      1
6chr1    778350    778907   40.5026129   40.5026129    73     1      1
7chr1    827085    827736   15.6656467   15.6656467    33     1      1
8chr1    869749    870148   10.0689882   10.0689882    13     1      1
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

Kind Regards 

Lauma 

DiffBind • 677 views
ADD COMMENT

Login before adding your answer.

Traffic: 765 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6