Hello

So am trying to do a heatmap for my RNA seq data.  My RPKM range is 0.03-1253. the weird thing is that the heat map is showing a value of 1 RPKM as high red color. So, there seems to be a mistake in the accuracy of the z-score. Also,if some of my values are similar e.g a gene has RPKM values are 18.13 , 18.26 and 19.21; however in the heatmap u see it as blue and red indicating that its very different. I could not figure what went wrong. I would appreciate any suggestions

> install.packages("gplots")
> library("Heatplus") 

> library("RColorBrewer") 

> RPKM=read.csv("marker.csv", row.names = 1) 
> RPKM_MATRIX=as.matrix(t(RPKM))
 > color_palette=colorRampPalette(c("blue","red"))(n=299) 
> heatmap.2(RPKM_MATRIX,main="DESeq",trace="none",margins=c(10,12),scale="col",col=color_palette,dendrogram="none")

> sessionInfo() 
R version 3.5.0 (2018-04-23)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows >= 8 x64 (build 9200)

Matrix products: default

locale:
[1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252   
[3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C                          
[5] LC_TIME=English_United States.1252    

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] RColorBrewer_1.1-2 Heatplus_2.26.0    gplots_3.0.1      

loaded via a namespace (and not attached):
[1] compiler_3.5.0     tools_3.5.0        KernSmooth_2.23-15 gdata_2.18.0       caTools_1.17.1    
[6] bitops_1.0-6       gtools_3.8.1

gplots is not a Bioconductor package. Please ask your question in a more appropriate forum, e.g., R-help.