Hello everybody,
My question isn't actually about the use of the package, since I've already used it successfully for a qPCR array with 96 genes. I was wondering if someone knows about a couple of papers where limma is used to assess DEGs in a qPCR array and not on another microarray platform or RNA seq. I know it's perfectly possible to use it this way, as the manual states, and I've actually read some questions in Bioconductor regarding that, but I'm having a really hard time finding the aforementioned literature. All I've found so far are works where limma is used upon another technology and the qPCR is only used to confirm DEGs, but not limma being used on the qPCR itself.
I'd really appreciate some help here and I apologize beforehand in case this ilk of questions is not very well suited for this place.
Best regards,
Daniel.
Thank you very much, Mr. Smith. I seize this chance to sincerely thank you for your valuable package.
Regards.