How to get peaks summit using dba.peakset
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Udi Landau ▴ 30
@udi-landau-9726
Last seen 4.9 years ago

Hi,

I cannot find the way to get the coordination for the summits of my peaks.

I added the summit option to the dba.count and I am looking at the dba.peakset documentation and cannot figure out how to get the data..

Is there a way to get the summits of the peaks together with the differential affinity data when using dba.report?

Thanks

diffbind • 1.0k views
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Rory Stark ★ 5.2k
@rory-stark-5741
Last seen 8 weeks ago
Cambridge, UK

You can get the summit coordinates for the full binding matrix by setting the score to DBA_SCORE_SUMMIT_POS. Here's how to do it using the example dataset:

data(tamoxifen_analysis)
tamoxifen <- dba.count(tamoxifen, peaks=NULL, score=DBA_SCORE_SUMMIT_POS)
summitCoordinates <- dba.peakset(tamoxifen, bRetrieve=TRUE)

If you want the summits for the differentially bound sites only, here's a snippet that will accomplish that:

DBreport <- dba.report(tamoxifen)
DBsites <- as.integer(names(DBreport))
summitCoordinates[DBsites,]

 

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Thank you for your quick reply! 

I see that I get the summits for each replicate.

How can I get the summit of the consensus peaks?.

I tried to add the argument 'consensus = -DBA_REPLICATE' to the dba.peakset command but it didn't change the output. 

When I think of it.. is there one peak that represent all the replicates or am I getting it wrong?

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