mouse 430_2 error message
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@dmitry-grigoryev-234
Last seen 9.7 years ago
Hi, I'm running rma on Affymetrix Mouse430_2 and getting this message: Error in density(x, kernel = "epanechnikov", n = 2^14) : need at least 2 points to select a bandwidth automatically Any suggestions? Note that gcrma runs just fine. Thank you Dmitry
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@ting-yuan-liu-fhcrc-1221
Last seen 9.7 years ago
Hi Dmitry, Based on the error message you provided, it is difficult to understand what happened and to provide help on this. Could you provide detail information such as (1) the codes you are running before you get this error (2) the output of sessionInfo() These kinds of information will be much helpful than the error messages only. HTH, Ting-Yuan ______________________________________ Ting-Yuan Liu Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center Seattle, WA, USA ______________________________________ On Fri, 3 Feb 2006, Dmitry wrote: > Hi, > > I'm running rma on Affymetrix Mouse430_2 and getting this message: > > Error in density(x, kernel = "epanechnikov", n = 2^14) : > need at least 2 points to select a bandwidth automatically > > Any suggestions? > > Note that gcrma runs just fine. > > Thank you > > Dmitry > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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I run the basic code. Here is the whole thing. > library(affy) Loading required package: Biobase Loading required package: tools Welcome to Bioconductor Vignettes contain introductory material. To view, simply type 'openVignette()' or start with 'help(Biobase)'. For details on reading vignettes, see the openVignette help page. > D<-ReadAffy() > e<-rma(D) Background correcting Error in density.default(x, kernel = "epanechnikov", n = 2^14) : need at least 2 points to select a bandwidth automatically > -----Original Message----- From: Ting-Yuan Liu [mailto:tliu@fhcrc.org] Sent: Friday, February 03, 2006 1:21 PM To: Dmitry Cc: bioconductor at stat.math.ethz.ch Subject: Re: [BioC] mouse 430_2 error message Hi Dmitry, Based on the error message you provided, it is difficult to understand what happened and to provide help on this. Could you provide detail information such as (1) the codes you are running before you get this error (2) the output of sessionInfo() These kinds of information will be much helpful than the error messages only. HTH, Ting-Yuan ______________________________________ Ting-Yuan Liu Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center Seattle, WA, USA ______________________________________ On Fri, 3 Feb 2006, Dmitry wrote: > Hi, > > I'm running rma on Affymetrix Mouse430_2 and getting this message: > > Error in density(x, kernel = "epanechnikov", n = 2^14) : > need at least 2 points to select a bandwidth automatically > > Any suggestions? > > Note that gcrma runs just fine. > > Thank you > > Dmitry > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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Typically when I have encountered others who have had this error occur it is because they have corrupted data. For instance this piece of demonstration code will generate the same error: library(affy);library(affydata) data(Dilution) Dilution.Corrupted <- Dilution pm(Dilution.Corrupted)[1,1] <- 30000000 # that is an extreme value outside the # range of normal raw probe intensities eset <- rma(Dilution.Corrupted) My suggestion would be to examine things along those lines. On Fri, 2006-02-03 at 13:29 -0500, Dmitry wrote: > I run the basic code. > Here is the whole thing. > > > library(affy) > Loading required package: Biobase > Loading required package: tools > > Welcome to Bioconductor > > Vignettes contain introductory material. > To view, simply type 'openVignette()' or start with 'help(Biobase)'. > > For details on reading vignettes, see the openVignette help page. > > > D<-ReadAffy() > > e<-rma(D) > Background correcting > Error in density.default(x, kernel = "epanechnikov", n = 2^14) : > need at least 2 points to select a bandwidth automatically > >
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