Hello all,

I am working on Trinity for denovo assembly of my data but stuck at differential gene expression step(screenshot). I have 3 sample files and followed the steps as mentioned in the trinity tutorial but somehow getting this error in edgeR.

Error, cmd: R --vanilla -q < matrix.counts.matrix.align_estimate_rsem_P3_vs_align_estimate_rsem_unP3.align_estimate_rsem_unP3.vs.align_estimate_rsem_unP3. EdgeR.Rscript died with ret (32512) at /app/setup/trinityrnaseq-2.2.0/analysis/diffenetialexpression/run_DE_analysis.pl     line 712.

WARNING: This EdgeR comparison failed...

Plz, guide me.

Best Regards, Sakshi

You are using a Perl script that the Trinity author created, which just happens to call some functions in edgeR, so your question really has to do with that Perl script rather than edgeR. Rather than asking here, you should be asking the authors of Trinity on their Google Groups page.