Please help me to use the function named read.agiMicroRna(). Getting the error given below.

> library("AgiMicroRna")
> dd=AgiMicroRna:::read.agiMicroRna(targets.micro,
+                     columns=list(TGS="gTotalGeneSignal",
+                                  TPS="gTotalProbeSignal",
+                                  meanS="gMeanSignal",
+                                  procS="gProcessedSignal"),
+                     other.columns=list(IsGeneDetected="gIsGeneDetected",
+                                        IsSaturated="gIsSaturated",
+                                        IsFeatNonUnifOF="gIsFeatNonUnifOL",
+                                        IsFeatPopnOL="gIsFeatPopnOL",
+                                        BGKmd="gBGMedianSignal"),
+                     annotation = c( "ControlType", "ProbeName","SystematicName"),
+                     verbose=TRUE)
reading file  1  -  257015615988_1_1.txt 
Error in `[.data.frame`(obj, , columns[[a]]) : undefined columns selected

Dear topijush,

please check your input file 25701561598811.txt and make sure that it contains the columns you're providing to the function in arguments columns, other.columns, and annotation -- the error message is trying to tell you that at least one of these cannot be found.

BTW, your message is a bit hard to read for potential helpers, isn't it?

Hope this helps.