Hey, I'm french student and it's my first mail on bioconductor, I just want to use the loess normalization on my data, but i don't understand which type of data the function "normaliseWithinArrays" want. And, if i must apply the function log, before or after normalisation. So i'm a little lost. (no log) (no background) (raw data) my data profile : dat$R dat$G 49 35 118 34 29 101.5 42 46.5 44 287.5 31 174 my script : MA <- normalizeWithinArrays(don, method="loess") thinks, Sylvain Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34 ?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn)

On 4/24/06 1:19 PM, "sysra" <sysra at="" laposte.net=""> wrote: > Hey, > > I'm french student and it's my first mail on bioconductor, > > I just want to use the loess normalization on my data, but i > don't understand which type of data the function > "normaliseWithinArrays" want. And, if i must apply the > function log, before or after normalisation. I think reading the section 6 (preprocessing of two-color data) of the limma user guide would probably be helpful. Also, read the help for the normalizeWithinArrays command by typing: help('normalizeWithinArrays') Sean

Hey, I'm french student and it's my first mail on bioconductor, I just want to use the loess normalization on my data, but i don't understand which type of data the function "normaliseWithinArrays" want. And, if i must apply the function log, before or after normalisation. So i'm a little lost. (no log) (no background) (raw data) my data profile : dat$R dat$G 49 35 118 34 29 101.5 42 46.5 44 287.5 31 174 my script : MA <- normalizeWithinArrays(don, method="loess") thinks, Sylvain Acc?dez au courrier ?lectronique de La Poste : www.laposte.net ; 3615 LAPOSTENET (0,34 ?/mn) ; t?l : 08 92 68 13 50 (0,34?/mn)