Hi everyone, I am using the rflowcyt package to analyze data generated on a FACSAria machine with the DiVa software. I collect data for 10 minutes after an initial stimulation of the cells than I export the FCS 3.0 files to rflowcyt. After reading in the data I noticed that the data on channel "Time" do not correspond to the actual duration of the measurement. Namely, when I collected data for 10 minutes (=600 sec), my time-scale in rflowcyt spans to 250 "units", instead of 600. (I am calculating a lot of statistics, thus the proper time-resolution would be very important.) However, if I try to analyze the same data with another software (e. g. FlowJo), the scale is correct, thus the data needed are within the file, simply I am not able to extract it. What should I do? How can I get the proper time-resolution? My second question: I have never been able to read in my FCS3.0 file from the DiVa software via the prada package. Does prada recognize the DiVa- derived FCS3.0 files? If not, do you have an extension for it? Your help is greatly appreciated, best Andras Andras Treszl MD PhD First Department of Pediatrics, Semmelweis University, Budapest, Hungary B?kay u. 53 H-1083 Budapest, Hungary treszl at gyer1.sote.hu Tel.: 36/1/334-3186 Fax.: 36/1/313-8212

Hi Andras, Maybe you can send us (Florian Hahne and myself) one of your time serie FCS file? We will try to solve your problems. Best, Nolwenn ************************************** Nolwenn Le Meur, PhD Fred Hutchinson Cancer Research Center Computational Biology 1100 Fairview Ave. N., M2-B876 P.O. Box 19024 Seattle, WA 98109-1024 On Thu, 25 May 2006, dr. Treszl Andr?s wrote: > Hi everyone, > > I am using the rflowcyt package to analyze data generated on a FACSAria > machine with the DiVa software. I collect data for 10 minutes after an > initial stimulation of the cells than I export the FCS 3.0 files to > rflowcyt. After reading in the data I noticed that the data on > channel "Time" do not correspond to the actual duration of the measurement. > Namely, when I collected data for 10 minutes (=600 sec), my time- scale in > rflowcyt spans to 250 "units", instead of 600. (I am calculating a lot of > statistics, thus the proper time-resolution would be very important.) > However, if I try to analyze the same data with another software (e. g. > FlowJo), the scale is correct, thus the data needed are within the file, > simply I am not able to extract it. What should I do? How can I get the > proper time-resolution? > > My second question: I have never been able to read in my FCS3.0 file from > the DiVa software via the prada package. Does prada recognize the DiVa- > derived FCS3.0 files? If not, do you have an extension for it? > > Your help is greatly appreciated, > > best > > Andras > > Andras Treszl MD PhD > First Department of Pediatrics, Semmelweis University, Budapest, Hungary > B?kay u. 53 > H-1083 Budapest, Hungary > treszl at gyer1.sote.hu > Tel.: 36/1/334-3186 > Fax.: 36/1/313-8212 > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >