Detailed explanations for DEXSeq output columns
1
0
Entering edit mode
@angel-liang-23540
Last seen 4.4 years ago

Hi, I have output results from DEXSeq using the DEXSeqDataSetFromHTSeq() method, but I can't find any detailed documentation which explains what the column names mean:

DEXSeqresults <- DEXSeqResults(DEXSeqDataSet) colnames(DEXSeqresults)

[1] "groupID" "featureID"
"exonBaseMean" "dispersion"
[5] "stat" "pvalue"
"padj" "control"
[9] "treat" "log2foldtreatcontrol" "genomicData.seqnames" "genomicData.start"
[13] "genomicData.end" "genomicData.width" "genomicData.strand" "countData.1"
[17] "countData.2" "countData.3"
"countData.4" "countData.5"
[21] "countData.6" "transcripts"
"comparison"

It's obvious that countData.x columns mean the raw input counts, but what about the "control" and "treat" columns for example? could they be logCPM values? what about "stat" and "exonBaseMean"?

Thanks in advance

DEXSeq • 1.4k views
ADD COMMENT
1
Entering edit mode
Alejandro Reyes ★ 1.9k
@alejandro-reyes-5124
Last seen 5 months ago
Novartis Institutes for BioMedical Reseā€¦

Thanks for your question Angel. You can find the details of each column in the metadata of the columns:

data(pasillaDEXSeqDataSet, package="pasilla")
dxd <- estimateSizeFactors( dxd )
dxd <- estimateDispersions( dxd )
dxd <- testForDEU( dxd )
dxr <- DEXSeqResults( dxd )
elementMetadata(dxr)

ADD COMMENT
0
Entering edit mode

thanks so much! i didn't know you could do that!

ADD REPLY

Login before adding your answer.

Traffic: 949 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6