Hi list, I have a set of cDNA slides where the where the spotting medium was prepared in two batches and one batch is causing auto-flourescence in the green channel for one region of each grid. I want to see if I can recover anything from this by doing separate loess normalization for the two regions -- can anyone point me in the right direction for doing this? bye rob -- Rob Dunne Fax: +61 2 9325 3200 Tel: +61 2 9325 3263 CSIRO Mathematical and Information Sciences +61 2 9325 3100 Locked Bag 17, North Ryde, New South Wales, Australia, 1670 http://matilda.vu.edu.au/~dunne Email: Rob.Dunne@csiro.au Java has certainly revolutionized marketing and litigation.